1. iGEM 2010 General Update and Project Ideas

2. iGEM 2010 iGEM 2010 registration is open
Recruitment and forming a Team
Saftey Lab Training on Saturdays
Synthetic Biology bootcamp, 1st week of summer
Informal Lectures
Funding
Publicity / Outreach
EOH
Biobricks
Public Engagement Symposium
Surveys
Undergraduate Research Symposium
IBE Conference March 4-6

3. Advisor Involvement Weekly meetings over the summer Critique ideas
Recommend papers
Informal Lectures during the beginning of summer
Ex: How to build primers

4. General Project Goals Gold!
It'd be nice to build off of the sRNA we've already been working with.
The ultimate goal should involve some concrete humanitarian application
Keep the different category prizes in mind when deciding on a project.

5. Project Ideas Make a decoder More with logic gates Maz proofing
Make a decoder
More with logic gates
Maz proofing
 Test the Tools Team's Program
Gene Silencing through sRNAs

6. Decoder and Logic Gates
Take the Decoder to the next level by having it respond to toxins and produce either a reporter or anti-toxin.
This would give us the opportunity to have a really humanitarian application
Create a system with different logic gates
Have a system loop back on itself
Submit specific logic gates for future teams to use

7. Test the Tools Team Project
Create and test one of the pathways that their program

8. mazEF Programming Cell Death
Toxin-Antitoxin system
mazE antitoxin
mazF toxin
Responds to stresses
specific antibiotics
EDF (Extracellular Death Factor) Quorum sensing molecules 

9. Gene silencing in Prokaryotes Prokaryotes as machines
Issues
Gene silencing in Prokaryotes
Prokaryotes as machines

10. Issues: Gene Silencing in Prokaryotes
RNAi has proven to be extremely useful in eukaryotes. It has applications in just about every field of biology, medicine, and more.
No standardized non-invasive artificial gene silencing exists for prokaryotes.

11. Issues: Prokaryotes as Machines
~50% of dry cell mass is protein.
Peptide Synthesis is the most taxing on cellular resources.
If proteins are not the output or desired product, using other means of performing the same function will make the machine more efficient and conservative.

12. Artificial RNA-RNA Regulation
sRNA Model
Endogenously used as regulators in response to cellular stress
Target Specificity
Degradation of Transcript
Irreversible
Transient, Dynamic
Recent Characterizations
Structure
Function
Origin
Associated Proteins
Performance

13. Artificial RNA-RNA Regulation
Transcript Stability and Regulation
Construct high stability transcripts
Construct strong RNA regulators
Sites of Strong Regulation
RBS
Stem-loop of Instrinsic Terminator
A/U rich regions

14. Artificial RNA-RNA Regulation
Gene Silencing as Genetic Tools
Plasmid Construct
MegaPrimer P1 P2
Target Specificity Hfq Binding Intrinsic Terminator
High Copy #