1. Reductions in claudin-1 may enhance susceptibility to herpes simplex virus 1 infections in atopic dermatitis 
Anna De Benedetto, MD, Mark K. Slifka, PhD, Nicholas M. Rafaels, MS, I-Hsin Kuo, MS, Steve N. Georas, MD, Mark Boguniewicz, MD, Tissa Hata, MD, Lynda C. Schneider, MD, Jon M. Hanifin, MD, Richard L. Gallo, MD, David C. Johnson, PhD, Kathleen C. Barnes, PhD, Donald Y.M. Leung, MD, PhD, Lisa A. Beck, MD 
Journal of Allergy and Clinical Immunology 
Volume 128, Issue 1, Pages e5 (July 2011)
DOI: /j.jaci
Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2. Fig 1
Knockdown of claudin-1 (CLDN1) in human keratinocytes increases HSV-1 infectivity. Green indicates HSV-1 staining. DAPI (blue) nuclear stain provides an assessment of cell density (bars = 200 μm). Larger and more numerous FFUs were detected in PHKs treated with CLDN1 siRNA (A) compared with the control siRNA (B; representative images of n = 6 experiments). Cells stained with a rabbit IgG isotype control were all negative (data not shown). C, An average of 4.8 ± 0.7 FFUs/field were counted in CLDN1 knockdown PHKs and 2.5 ± 0.5 FFUs/field in the control samples (*P = .05; n = 6). D, The diameter of the major axis of FFUs was significantly greater in PHKs whose CLDN1 expression was reduced (207 ± 21 μm) compared with controls (152 ± 40 μm; *P = .03; n = 6). E, Similar observations were noted in FFU area (*P = .04; n = 6) in CLDN1 knockdown (15,649 ± 4367 pixels [px]) compared with control transfection (9902 ± 4943 pixels).
Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci )
Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3. Fig 2
Excluding subjects with the 2 most common FLG mutations significantly enhanced CLDN1 associations with EH. An SNP (rs ; P = .03) was significantly associated with EH in the European American population. When subjects were stratified for FLG (eg, without [w/o] FLG mutations), the association was slightly more significant (P = .02). In this group, we observed 1 additional intronic SNP that was significantly associated with EH (rs ; P = .001). In the African American population, an SNP (rs ; P = .040) in the promoter region was associated with EH. w/, With.
Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci )
Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4. Knockdown of CLDN1 in human keratinocytes increases HSV-1 infectivity
Knockdown of CLDN1 in human keratinocytes increases HSV-1 infectivity. Infectious center assay demonstrates that CLDN1 knockdown PHKs were more infected with HSV-1 (38 ± 6.43%; n = 3) than control transfected cells (28 ± 7%; n = 3; **P = .003).
Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci )
Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5. Silencing of CLDN1 does not affect expression of nectin-1 (PVRL1)
Silencing of CLDN1 does not affect expression of nectin-1 (PVRL1). CLDN1 siRNA (100 nmol/L) resulted in a 50% reduction in CLDN1 transcripts compared to control transfected cells (0.5 ± 0.6% fold; *P = .5 × 10−6; n = 5/group). There was no effect on mRNA expression of nectin-1 (PVRL1; cldn-1 siRNA, 0.99 ± 0.16; control, 1.1 ± 0.14; n = 5/group). Relative gene expressions were calculated by using the 2-ΔΔCt method, in which Ct indicates cycle threshold, the fractional cycle number where the fluorescent signal reaches detection threshold. The normalized Ct value of each sample was calculated by using GAPDH.
Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci )
Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions