1. The dicBF chromosomal locus and roles of DicF and DicB in cell physiology.
The dicBF chromosomal locus and roles of DicF and DicB in cell physiology. (A) Cartoon illustrating the chromosomal arrangement of the dicBF locus with the specific deletions depicted. A Plac promoter was used to replace the native dicBF promoter (F+B+) in an otherwise wt strain. Gene dicF or gene dicB was deleted in this context to create an F−B+ strain or an F+B− strain, respectively. Both genes were mutated in this context to make an F−B− strain. (B) The wild-type strain (WT) and the four constructs described for panel A were streaked on LB plates with or without 0.5 mM IPTG. (C) The strains described above were grown in liquid LB medium, 0.5 mM IPTG was added at time 0, and growth was monitored over time. (D) The viability of the strains described for panel C was measured at the time points indicated by asterisks.
Divya Balasubramanian et al. mSystems 2016; doi: /mSystems