1. Volume 132, Issue 2, Pages 615-627 (February 2007)
The Vanilloid Receptor Initiates and Maintains Colonic Hypersensitivity Induced by Neonatal Colon Irritation in Rats 
John Winston, Mohan Shenoy, Dylan Medley, Ashutosh Naniwadekar, Pankaj Jay Pasricha 
Gastroenterology 
Volume 132, Issue 2, Pages (February 2007)
DOI: /j.gastro
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2. Figure 1
Effect of neonatal P10 acetic acid treatment on sensitivity to CRD when measured at 8 weeks of age. (A) AWR scores as a function of distention pressure; P10 saline treated, n = 9; P10 acetic acid treated, n = 8. ***P = .009, **P = .023, #P = .014, ##P = .03. (B) EMG activity in the external oblique muscle in response to graded CRD. EMG is expressed in units of V/s; each point represents the mean of 17 rats from 3 independent experiments (neonatal treatment, F = 5.288, P = .030; distention pressure, F = 382, P < .001, 2-way repeated-measures ANOVA). **P = .010, *P = .035.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2007 AGA Institute Terms and Conditions

3. Figure 2
Effect of colonic treatment with 5% acetic acid at 8 weeks on sensitivity to CRD at 12 weeks. AWR scores as a function of distention pressure, n = 5. A significant increase in AWR grade in response to increasing CRD pressure was observed in both P10 acetic acid– and P10 saline-treated rats (P < .001). Mann–Whitney rank sum test at each distention pressure found no significant differences between treatment groups (50 mm Hg, P = .690; 60 mm Hg, P = .421; 70 mm Hg, P = .310; 80 mm Hg, P = .69).
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2007 AGA Institute Terms and Conditions

4. Figure 3
Effect of P10 acetic acid treatment on colons of neonatal and adult rats. (A–D) Photomicrographs of H&E-stained sections from colons of neonatal rats. (A and C) Saline and (B and D) acetic acid treatment at 4 hours. (A–D) Twenty-four hours after treatment. (E–J) Photomicrographs of H&E-stained sections from (E and F) proximal, (G and H) middle, and (I and J) distal colon from (E, G, and I) P10 saline- and (F, H, and J) P10 acetic acid–treated rats.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2007 AGA Institute Terms and Conditions

5. Figure 4
Effect of neonatal P10 capsaicin treatment on sensitivity to CRD when measured at 7 weeks of age. Neonatal treatment with capsaicin resulted in increased sensitivity of these rats to CRD compared with vehicle-treated or unmanipulated rats (n = 5). *P = .007, **P < .001.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2007 AGA Institute Terms and Conditions

6. Figure 5
Effect of pretreatment with TRPV1 antagonist SB-366,791 (1.3 mg/kg subcutaneously) before acetic acid infusion in 10-day-old pups on the sensitivity of these rats to graded CRD when they were tested at 8 weeks of age (n = 10). (A) AWR scores as a function of distention pressure: P10 acetic acid vehicle versus P10 acetic acid SB-366,791: *P < .05; P10 acetic acid vehicle versus P10 saline vehicle: #P < .05. (B) EMG activity (V/s) in the external oblique muscle in response to graded CRD. P10 acetic acid vehicle versus P10 acetic acid SB-366,791: ***P = .002, *P = .034, **P < .03. P10 acetic acid vehicle versus P10 saline vehicle: +P = .025, ++P < .001.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2007 AGA Institute Terms and Conditions

7. Figure 6
Pretreatment with TRPV1 antagonists (A and B) SB-366,791 (2.6 mg/kg subcutaneously) or (C and D) I-RTX 2 μmol/kg before acetic acid infusion in 10-day-old pups reduced sensitivity of these rats to graded CRD when tested at 8 weeks of age (n = 10). (A) SB-366,791, AWR grade: ****P = .001, ***P = .002, **P = .003, *P = (B) SB-366,791, EMG: *P = .050, **P = .038, ***P = (C) I-RTX, AWR grade: ***P = .002, **P = .037, *P = .05. (D) I-RTX, EMG: ###P < .001, ##P = .003, #P = .047.
Gastroenterology  , DOI: ( /j.gastro )
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8. Figure 7
Treatment with TRPV1 antagonist SB reduced sensitivity to CRD in P10 acetic acid–treated rats when tested at 7–8 weeks of age (n = 10). P10 acetic acid–treated rats treated with SB-366,791 compared with P10 acetic acid–treated rats treated with vehicle, *P = .023, **P = P10 acetic acid–treated rats treated with vehicle compared with P10 saline-treated rats with vehicle, *P = .042, **P = .009.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2007 AGA Institute Terms and Conditions

9. Figure 8
TRPV1 expression in adult rats. (A) Western blots showing TRPV1 protein expression in LS DRG (L6, S1) and TL DRG (T13, L1, L2) of adult rats (**P < .01, *P < .03). (B) Western blots showing TRPV1 protein expression in LS and TL spinal cord of adult rats. (C) TRPV1 immunofluorescence (green) in DiI-labeled (red) colon afferents in sections from LS and TL DRG. Open arrows designate neurons showing colocalization between TRPV1 and DiI; thin arrows mark DiI-labeled neurons in which TRPV1 immunofluorescence was not detected. (D) Bar graphs showing fold change in TRPV1 messenger RNA in TL and LS DRG in adult rats that were neonatally sensitized with acetic acid compared with controls (*P < .05).
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2007 AGA Institute Terms and Conditions