1. Golden Gate assembly of transposon delivery vectors from part vectors.
Golden Gate assembly of transposon delivery vectors from part vectors. (A to E) The five part vectors that are used for Golden Gate assembly of the magic pool transposon delivery vectors: part1 vector, part2 vector, part3 vector, barcoded part4 vector, and part5 vector (not drawn to scale). We show the sequences of the 4-nucleotide overhangs for Golden Gate assembly. (F) The transposon delivery vector with DNA barcode. ColE1 is the replication origin ColE1. cat is the chloramphenicol resistance gene. GFP is green fluorescent protein. oriT is the origin of transfer. AmpR is the beta-lactam resistance gene. R6K is a conditional replication origin. N20 is random 20-nucleotide DNA barcode.
Hualan Liu et al. mSystems 2018; doi: /mSystems