1. A Pixel-Encoder Retinal Ganglion Cell with Spatially Offset Excitatory and Inhibitory Receptive Fields 
Keith P. Johnson, Lei Zhao, Daniel Kerschensteiner 
Cell Reports 
Volume 22, Issue 6, Pages (February 2018)
DOI: /j.celrep
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2. Cell Reports 2018 22, 1462-1472DOI: (10.1016/j.celrep.2018.01.037)
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3. Figure 1 Morphology and Light Responses of PixON-RGCs
(A) Orthogonal projections of a two-photon image stack through a representative PixON-RGC filled during physiological recording in Grik4-Cre:Ai9 mice.
(B) Length of the longest axis through a polygon around the PixON dendritic field (n = 38), equivalent diameter of the PixON-RGC dendritic field (n = 38), and total dendritic length (n = 23) of PixON-RGCs.
(C) Sholl analysis of traced PixON-RGC dendrites (n = 23).
(D) Stratification of PixON-RGC dendrites within the IPL (n = 18; 0%–100% border between inner plexiform layer and inner nuclear layer to border between inner plexiform layer and ganglion cell layer). In (C) and (D), lines (shaded areas) indicate the means (±SEMs) of the traced population.
(E) Representative spiking (black), and excitatory (red) and inhibitory (cyan) currents in response to presentation of a 300-μm circle (2 s ON, 2 s OFF; 1,500 R∗/rod/s background) centered on the soma of the recorded cell. Dashed lines show baselines in the absence of stimulus.
(F) Spontaneous and peak firing rates (n = 23) in response to the same stimulus as in (E).
(G) Percentage of peak spike response remaining 1.5 s after stimulus onset (n = 23).
(H) Change in excitatory and inhibitory conductance (n = 38) in response to the same stimulus as in (E).
(I) Percentage of peak excitatory conductance remaining 1.5 s after stimulus onset (n = 38).
In (F)–(I), dots represent data of individual cells, whereas larger circles (error bars) indicate means (±SEMs) of the respective populations. See also Figures S1, S2, and S4.
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4. Figure 2
Linear Contrast Response Functions and Linear Spatial Integration of PixON-RGCs
(A and C) Representative spike (A) and EPSC (C) responses to contrast steps presented in a 300-μm circle centered on the soma of the recorded cell.
(B and D) Contrast response functions of normalized spike rate (B, n = 13) and excitation (D, n = 6). Circles (error bars) indicate means (±SEMs) of the respective populations.
(E) Representative texture stimuli of different spatial scales masked in a 300-μm circle. Textures appeared from a gray background (onset) and were then translated 33 μm in either the dorsal, ventral, nasal, or temporal direction (motion). Textured regions were of the same mean luminance as the background.
(F and I) Representative spike (F) and excitatory (I) responses of a PixON-RGC to presentation of textures of the spatial scales shown in (E).
(G and H) Summary data of PixON-RGC spike responses to the onset (G) and motion (H) of texture stimuli of different spatial scales. Circles (error bars) indicate means (±SEMs) of the population (n = 13).
(J and K) Analogous to (G) and (H), respectively, for excitation (n = 3).
See also Figures S3–S5.
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5. Figure 3 Spatial receptive fields of PixON-RGCs
(A, C, and E) Representative spike (A, black), EPSC (C, red), and IPSC (E, cyan) responses to square-wave modulation (2 s ON, 2 s OFF) of circles of varying diameter. Dashed lines show baselines in the absence of stimulus.
(B, D, and F) Firing rates (B, n = 22) and changes in excitatory (D, n = 9) and inhibitory conductances (F, n = 9) during ON (open circles) and OFF (filled circles) plotted as a function of stimulus diameter. Circles (error bars) indicate means (±SEMs) of the respective populations.
See also Figure S4.
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6. Figure 4 Kinetics and Mechanisms of PixON-RGC Inhibition
(A) Representative normalized excitatory (red) and inhibitory (cyan) conductances in response to presentation of a 1,200-μm circle.
(B) Time from stimulus onset to peak of excitatory (n = 15) and inhibitory (n = 15) currents in response to presentation of a 1,200-μm circle (p = 0.544).
(C) Representative IPSCs in control (cyan) solution and in the presence of the GABAA receptor antagonist gabazine (blue) evoked by square-wave modulation (2 s ON, 2 s OFF) of a 1,200-μm circle.
(D) Summary data of inhibitory conductance under control conditions (cyan) or in the presence of gabazine (blue, n = 4, p < 0.004). Lines indicate results from individual cells, whereas circles (error bars) indicate the means (±SEMs) of the respective population.
(E and F) Analogous to (C) and (D), respectively, but for tetrodotoxin (TTX, n = 4, p < 0.003).
(G and H) Analogous to (C) and (D), respectively, but for NMDA (D-AP5) and AMPA (NBQX) receptor antagonists (n = 3, p < 0.03).
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7. Figure 5 Spatially Offset Inhibition of PixON-RGCs
(A) Annuli of constant 1,200-μm outer diameter and varying inner diameter were presented as 100% contrast steps (2 s ON) from a gray background (1,500 R∗/rod/s).
(B) Representative IPSCs in response to annuli shown in (A).
(C) Summary data plotting inhibitory conductance as a function of the inner diameter of stimulus annuli. Circles (error bars) indicate mean (±SEM) of the recorded population (n = 7).
(D) The display was divided into a 3 × 3 grid of 300- × 300-μm squares and centered on the soma of the recorded cell. Between stimulus presentations all squares had the same luminance (1,500 R∗/rod/s). During stimulus presentation, one of the nine squares increased in luminance (100% contrast, 2 s ON).
(E) Representative IPSCs in response to appearance of squares in the positions shown in (A).
(F) Map of the normalized inhibitory conductance evoked by the appearance of a square at each position (n = 4). Coloration indicates the strength of inhibition.
(G) Sensitivity profiles of excitatory and inhibitory receptive fields in feedforward inhibition, in crossover inhibition, and in truly lateral inhibition.
See also Figures S6 and S7.
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8. Figure 6
Spatial Receptive Field Organization of PixON-RGCs with Green Stimulus
(A) Green visual stimulus (peak: 532 nm) was used to probe receptive field properties of PixON-RGCs.
(B) Schematic of the retina showing approximate locations of recorded cells (OTZ, opsin transition zone).
(C) Representative EPSC (red) and IPSC (cyan) responses to square-wave modulation (2 s ON, 2 s OFF) of either a 300- (left) or 1,200-μm (right) circle from a cell recorded in the dorsal retina. Dashed lines show baselines in the absence of stimulus.
(D) Summary of changes in excitatory and inhibitory conductances of cells recorded in the dorsal retina (n = 4) during the ON phase of the stimulus, plotted as a function of stimulus diameter. Circles (error bars) indicate means (±SEMs) of the respective populations.
(E–H) Analogous to (C) and (D), but for a cell recorded near the OTZ (E and F) (n = 1) or cells recorded in the ventral retina (G and H) (n = 3).
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9. Figure 7 Retrograde Tracing of PixON-RGCs’ Axonal Projections
(A) Representative cholera toxin subunit B (CTB) injection primarily in the core of dLGN.
(B) Morphology of a tdTomato and CTB positive (inset) cell targeted, recorded, and filled following injection of CTB into dLGN in a Grik4-Cre:Ai9 mouse.
(C) Percentage of recorded cells that were both tdTomato and CTB positive and had physiological and morphological properties of PixON-RGCs following CTB injection into different brain regions (dLGN: 19/65 cells; vLGN: 3/38 cells; SC: 1/14 cells; MTN: 0/10 cells).
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