1. An Immunoassay to Rapidly Measure Acetaminophen Protein Adducts Accurately Identifies Patients With Acute Liver Injury or Failure 
Dean W. Roberts, William M. Lee, Jack A. Hinson, Shasha Bai, Christopher J. Swearingen, R. Todd Stravitz, Adrian Reuben, Lynda Letzig, Pippa M. Simpson, Jody Rule, Robert J. Fontana, Daniel Ganger, K. Rajender Reddy, Iris Liou, Oren Fix, Laura P. James 
Clinical Gastroenterology and Hepatology 
Volume 15, Issue 4, Pages e3 (April 2017)
DOI: /j.cgh
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2. Figure 1
(A) AcetaSTAT test band intensity (amplitude) vs acetaminophen–protein adduct determinations by HPLC-EC, presented as the mean of 2 standard curves. (B) Archived readings and images of corresponding AcetaSTAT results.
Clinical Gastroenterology and Hepatology  , e3DOI: ( /j.cgh )
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3. Figure 2
AcetaSTAT test band amplitude vs acetaminophen–protein adduct determinations by HPLC-EC, presented as log base 10. The Y1 axis is 1/AcetaSTAT test band amplitude; the Y2 axis is the test band amplitude, presented as reflectance units. The cut-off point of 1200 arbitrary reflectance units at the AcetaSTAT test band is indicated by a horizontal dashed line. The vertical dashed line indicates the previously established diagnostic threshold of the HPLC-EC assay of 1.0 nmol APAP cys/mL,12 expressed as log base 10, or 0. Each symbol represents a study subject result, by diagnosis, for AcetaSTAT and HPLC-EC.
Clinical Gastroenterology and Hepatology  , e3DOI: ( /j.cgh )
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4. Supplementary Figure 1
(Top) Schematic and (bottom) image of AcetaSTAT test.
Clinical Gastroenterology and Hepatology  , e3DOI: ( /j.cgh )
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5. Supplementary Figure 2
Interference studies. Interference testing of the (A) AcetaSTAT test band and the (B) control band provided similar results in control serum (CS) and adduct serum (AS; 0.5 nmol/mL adduct). The addition of a drug mixture (D) to either CS or AS did not change the test band or control band amplitudes. Right: HPLC-EC quantitation for the corresponding groups. D contained hydrocodone, hydromorphone, codeine, diazepam, and clonazepam at 10× the high therapeutic level. These drugs commonly are combined with acetaminophen in prescription products or are commonly abused. V, vehicle. Mean ± SD shown, n = 8.
Clinical Gastroenterology and Hepatology  , e3DOI: ( /j.cgh )
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