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Nobuhisa Terada, MDa, Akiyoshi Konno, MDb, Kiyoshi Togawa, MDa 

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Presentation on theme: "Nobuhisa Terada, MDa, Akiyoshi Konno, MDb, Kiyoshi Togawa, MDa "— Presentation transcript:

1 Biochemical properties of eosinophils and their preferential accumulation mechanism in nasal allergy 
Nobuhisa Terada, MDa, Akiyoshi Konno, MDb, Kiyoshi Togawa, MDa  Journal of Allergy and Clinical Immunology  Volume 94, Issue 3, Pages (September 1994) DOI: / (94) Copyright © 1994 Mosby, Inc. Terms and Conditions

2 FIG. 1 Time schedule for study.
Journal of Allergy and Clinical Immunology  , DOI: ( / (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

3 FIG. 2 Changes in nasal symptoms after antigen challenge. A, Frequency of sneezing. B, Amount of nasal discharge. C, Percentage change in NAR. Vertical bars mean ± SE. Asterisks indicate significant change at each hour compared with baseline wash (0) performed before antigen challenge. Journal of Allergy and Clinical Immunology  , DOI: ( / (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

4 FIG. 2 Changes in nasal symptoms after antigen challenge. A, Frequency of sneezing. B, Amount of nasal discharge. C, Percentage change in NAR. Vertical bars mean ± SE. Asterisks indicate significant change at each hour compared with baseline wash (0) performed before antigen challenge. Journal of Allergy and Clinical Immunology  , DOI: ( / (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

5 FIG. 2 Changes in nasal symptoms after antigen challenge. A, Frequency of sneezing. B, Amount of nasal discharge. C, Percentage change in NAR. Vertical bars mean ± SE. Asterisks indicate significant change at each hour compared with baseline wash (0) performed before antigen challenge. Journal of Allergy and Clinical Immunology  , DOI: ( / (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

6 FIG. 3 Changes in inflammatory cell counts in nasal lavage fluids. Eosinophils in precipitate were counted in a cell counting plates after staining with Hinkelman's stain, whereas basophilic and mononuclear cells were counted after staining with Osada-Tatai stain and Türk stain, respectively. MNCs, Mononuclear cells; basophils, basophilic cells. Journal of Allergy and Clinical Immunology  , DOI: ( / (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

7 FIG. 4 Changes in levels of chemical mediators in nasal lavage fluids. Vertical bars mean ± SE. Asterisks indicate significant change at each hour compared with baseline wash (0) performed before antigen challenge. Journal of Allergy and Clinical Immunology  , DOI: ( / (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

8 FIG. 5 Changes in percentage of EG2-positive eosinophils. Although incidence of EG2 positive was 19.5% before challenge, it increased to 42.0% at 8 hours, 47.5% at 10 hours, and 47.5% at 12 hours. Vertical bars mean ± SE. Asterisks indicate significant change at each hour compared with baseline wash (0) performed before antigen challenge. Journal of Allergy and Clinical Immunology  , DOI: ( / (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

9 FIG. 6 Changes in amount of ECP. Amount of ECP increased during both early and late phases but more markedly during late phase. Vertical bars mean ± SE. Asterisks indicate significant change at each hour compared with baseline wash (0) performed before antigen challenge. Journal of Allergy and Clinical Immunology  , DOI: ( / (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

10 FIG. 7 Number of activated eosinophils and ECP amount are compared between seven patients with late phase response and seven patients without marked late phase response. Values are mean ± SE. Both number of activated eosinophils and amount of ECP showed a tendency to increase in positive group. Journal of Allergy and Clinical Immunology  , DOI: ( / (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

11 FIG. 8 Changes in histamine threshold after antigen challenge.
Journal of Allergy and Clinical Immunology  , DOI: ( / (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

12 FIG. 9 Immunostaining patterns in nasal mucosa. (Original magnification ×200.) Eosinophils were identified with monoclonal antibody BMK-13. A, Control nasal mucosa exposed to 100 μl PBS containing 0.1% bovin serum albumin. B, Nasal mucosa exposed to 100 μl 10 μg/ml rhIL-5. Journal of Allergy and Clinical Immunology  , DOI: ( / (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

13 FIG. 10 A, Number of eosinophils and amount of ECP in nasal lavage fluids before and after application of rhIL-5 and PBS. Values are mean ± SE. B, Amount of sIgA, IgA, IgG, and IgM in nasal lavage fluids before and after application of rhIL-5 and PBS. Values are mean ± SE. Journal of Allergy and Clinical Immunology  , DOI: ( / (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

14 FIG. 10 A, Number of eosinophils and amount of ECP in nasal lavage fluids before and after application of rhIL-5 and PBS. Values are mean ± SE. B, Amount of sIgA, IgA, IgG, and IgM in nasal lavage fluids before and after application of rhIL-5 and PBS. Values are mean ± SE. Journal of Allergy and Clinical Immunology  , DOI: ( / (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

15 FIG. 11 Changes in amount of IL-5 in nasal lavage fluids before and after antigen challenge. Amount of IL-5 represents 40 ml of nasal lavage fluid. Vertical bars mean ± SE. Journal of Allergy and Clinical Immunology  , DOI: ( / (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

16 FIG. 12 Comparison of total number of eosinophils in nasal lavage fluids in IL-5–positive group, IL-5–negative group, and group in whom antigen challenge was not carried out. Total number of eosinophils in IL-5–positive group was higher than that in IL-5–negative group. Values are mean ± SE. Journal of Allergy and Clinical Immunology  , DOI: ( / (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

17 FIG. 13 Effect of rhIL-5 in ICAM-1 mRNA expression in nasal mucosa with nasal allergy and with nonallergic rhinitis. Asterisk indicates significant increase compared with unstimulated ICAM-1 mRNA expression in absence of rhIL-5 (p < 0.01). Journal of Allergy and Clinical Immunology  , DOI: ( / (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

18 FIG. 14 Serum level of sICAM-1 subjects with nasal allergy and normal individuals. Values are mean ± SD. Journal of Allergy and Clinical Immunology  , DOI: ( / (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

19 FIG. 15 Inhibitory effect of anti–VLA-4 monoclonal antibody on inflammatory cell infiltration in nasal mucosa caused by antigen challenge. Asterisk indicates significant change compared with untreated nasal mucosa. *p < 0.05, **p < Values are mean ± SE. Journal of Allergy and Clinical Immunology  , DOI: ( / (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

20 FIG. 15 Inhibitory effect of anti–VLA-4 monoclonal antibody on inflammatory cell infiltration in nasal mucosa caused by antigen challenge. Asterisk indicates significant change compared with untreated nasal mucosa. *p < 0.05, **p < Values are mean ± SE. Journal of Allergy and Clinical Immunology  , DOI: ( / (94) ) Copyright © 1994 Mosby, Inc. Terms and Conditions

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