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Detection of Expanded T Cell Clones in Skin Biopsy Samples of Patients with Lichen Sclerosus et Atrophicus by T Cell Receptor-γ Polymerase Chain Reaction.

Published byMonica Kristin Samuelsson Modified over 5 years ago

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Presentation on theme: "Detection of Expanded T Cell Clones in Skin Biopsy Samples of Patients with Lichen Sclerosus et Atrophicus by T Cell Receptor-γ Polymerase Chain Reaction."— Presentation transcript:

1 Detection of Expanded T Cell Clones in Skin Biopsy Samples of Patients with Lichen Sclerosus et Atrophicus by T Cell Receptor-γ Polymerase Chain Reaction Assays  Ansgar Lukowsky, J. Marcus Muche, Wolfram Sterry, Heike Audring  Journal of Investigative Dermatology  Volume 115, Issue 2, Pages (August 2000) DOI: /j x Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Hematoxylin-eosin staining, preputial LSA with strong lymphohistiocytic infiltrate, divided into an epidermotropic and a dermal part, both separated by a fibrosis zone. Scale bar: 100 μm. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Temperature gradient gel of TCR-γ PCR products of LSA skin samples. Lane 1, DNA molecular weight marker (HincII digest of φX 174 RF DNA); lanes 2, 6–9, polyclonal PCR products; lanes 3–5: clonal PCR products, appearing as sharp bands below the middle range of the gel (arrow), lane 10, PCR product of clonal control DNA (Jurkat T cell line). Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Genescan-fragment analysis on an ABI 373A sequencing gel. Monoclonal (upper profile) and polyclonal (lower profile) TCR-γ PCR product of LSA skin samples. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

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