1. BMDC-EV induce phenotypically different myeloid cell sub-populations.
BMDC-EV induce phenotypically different myeloid cell sub-populations. (A) Protein expression profiles of individual immune cells in imBMDC-EV- and maBMDC-EV–containing tissue areas 6 h and 24 h after injection. The upper panels (also analyzed in Figs 4 and 5) show the whole tissue areas, whereas the lower panels depict the MELC protein profile of individual cells from these areas. Individual cells were chosen randomly and assigned an ID number (cell ID). Cells were selected in areas where EV were concentrated (EVca; yellow demarcated area, upper panels) or less concentrated (EVpa; blue demarcated areas). The latter was determined by the immunoreactivity score (IRS) (Remmele & Stegner, 1987). Colored boxes were inserted for explanations in the main text. Scale bar represents 50 μm (upper panels) and 7.5 μm (lower panels). (B) Expression levels of indicated markers for each numbered cell (cell ID in [A]). For better understanding, protein expression was divided into three levels (no, low, and high expression), which were color-coded. (C, D) Relative presence of cells with triple marker combinations in imBMDC-EV- and maBMDC-EV–containing tissue areas shown in (A). Triple combinations of the most abundant markers (CD45, Ly6C, Ly6G, and F4-80) and CD169 (serving as internal control) were assessed by StrataQuest software as explained in Fig S8 and Table 1 and displayed in bar diagrams.
Stefan Schierer et al. LSA 2018;1:e
© 2018 Schierer et al.