1. L-selectin–dependent rolling adhesion to TNF-α–stimulated HCMEC is reduced by NaClO3 but not by neuraminidase.
L-selectin–dependent rolling adhesion to TNF-α–stimulated HCMEC is reduced by NaClO3 but not by neuraminidase. At 2.4 dyne/cm2, adhesion of L-selectin+ NALM-6-L cells to HCMEC is significantly enhanced by TNF-α stimulation of endothelial cells. This stimulated adhesion is completely blocked by anti–L-selectin MoAb LAM1-3 and cannot be observed in L-selectin− NALM-6 cells. This L-selectin dependent adhesion can be reduced to one third, if posttranslational sulfation reactions are inhibited by cultivation of HCMEC in the presence of NaClO3 . Neuraminidase treatment of HCMEC shows no effect on the adhesion behavior. Mean values ±SEM; *P < .05 versus positive control (first column).
A. Zakrzewicz et al. Blood 1997;89:
©1997 by American Society of Hematology