Presentation is loading. Please wait.

Presentation is loading. Please wait.

A Novel Semiquantitative Fluorescence-Based Multiplex Polymerase Chain Reaction Assay for Rapid Simultaneous Detection of Bacterial and Parasitic Pathogens.

Published byIvar Jansen Modified over 5 years ago

Similar presentations

Presentation on theme: "A Novel Semiquantitative Fluorescence-Based Multiplex Polymerase Chain Reaction Assay for Rapid Simultaneous Detection of Bacterial and Parasitic Pathogens."— Presentation transcript:

1 A Novel Semiquantitative Fluorescence-Based Multiplex Polymerase Chain Reaction Assay for Rapid Simultaneous Detection of Bacterial and Parasitic Pathogens from Blood  Angamuthu Selvapandiyan, Katie Stabler, Nasim A. Ansari, Stephen Kerby, Jenny Riemenschneider, Poonam Salotra, Robert Duncan, Hira L. Nakhasi  The Journal of Molecular Diagnostics  Volume 7, Issue 2, Pages (May 2005) DOI: /S (10) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 A: Melt derivative analysis of the multiplex fluorescence PCR showing the amplification of specific products of B. anthracis (Sterne) (BA) with Tm 82.2°C, L. donovani (LD) with Tm 86.1°C, Y. pseudotuberculosis (YP) with TM 87.3°C, and the internal human control (HS) with Tm 88.8°C. B: Agarose gel confirmation of the products of the multiplex PCR stained with ethidium bromide from A showing the amplified products of: LD, L. donovani; HS, human; YP, Y. pseudotuberculosis; and BA, B. anthracis (Sterne). The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 A: Melt derivative analysis of the multiplex PCR assay with human blood carrying a known number of spiked B. anthracis (Sterne) spores. B: Agarose gel confirmation of the products of the multiplex PCR showing the linear relationship between the logarithm of the number of spores spiked and the amount of amplified band intensity of B. anthracis (Sterne). C: Quantitation graph showing the linear relationship between the height of the fluorescence peak above baseline of the specific amplicons (y axis) and the logarithm of the number of bacterial or parasitic pathogen cells individually spiked in 200 μl of blood (x axis). BA, B. anthracis (Sterne); YP, Y. pseudotuberculosis; LD, L. donovani; a.v., arbitrary value. The data represent the means ± SD of three independent experiments. The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Download ppt "A Novel Semiquantitative Fluorescence-Based Multiplex Polymerase Chain Reaction Assay for Rapid Simultaneous Detection of Bacterial and Parasitic Pathogens."

Similar presentations

A.Selvapandiyan LBPUA, OBRR, CBER, FDA, Bethesda, MD July 13 2006 Multiplex fluorescence-based PCR assay to detect pathogens in blood Multiplex fluorescence-based.

A.Selvapandiyan LBPUA, OBRR, CBER, FDA, Bethesda, MD July Multiplex fluorescence-based PCR assay to detect pathogens in blood Multiplex fluorescence-based.
Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.
Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,

Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,
CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.

CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.
Quantitative Detection and Differentiation of Human Herpesvirus 6 Subtypes in Bone Marrow Transplant Patients by Using a Single Real-Time Polymerase Chain.

Quantitative Detection and Differentiation of Human Herpesvirus 6 Subtypes in Bone Marrow Transplant Patients by Using a Single Real-Time Polymerase Chain.
An Enhanced Polymerase Chain Reaction Assay to Detect Pre- and Full Mutation Alleles of the Fragile X Mental Retardation 1 Gene  Alessandro Saluto, Alessandro.

An Enhanced Polymerase Chain Reaction Assay to Detect Pre- and Full Mutation Alleles of the Fragile X Mental Retardation 1 Gene  Alessandro Saluto, Alessandro.
A Prospective Clinical Study on the Use of Reverse Transcription-Polymerase Chain Reaction for the Early Diagnosis of Dengue Fever  Kamaljit Singh, Ajit.

A Prospective Clinical Study on the Use of Reverse Transcription-Polymerase Chain Reaction for the Early Diagnosis of Dengue Fever  Kamaljit Singh, Ajit.
Large Pathogenic Expansions in the SCA2 and SCA7 Genes Can Be Detected by Fluorescent Repeat-Primed Polymerase Chain Reaction Assay  Claudia Cagnoli,

Large Pathogenic Expansions in the SCA2 and SCA7 Genes Can Be Detected by Fluorescent Repeat-Primed Polymerase Chain Reaction Assay  Claudia Cagnoli,
Todd S. Laughlin, Michael W. Becker, Jane L. Liesveld, Deborah A

Todd S. Laughlin, Michael W. Becker, Jane L. Liesveld, Deborah A
Locked Nucleic Acids Can Enhance the Analytical Performance of Quantitative Methylation-Specific Polymerase Chain Reaction  Karen S. Gustafson  The Journal.

Locked Nucleic Acids Can Enhance the Analytical Performance of Quantitative Methylation-Specific Polymerase Chain Reaction  Karen S. Gustafson  The Journal.
The Frequency of Immunoglobulin Heavy Chain Gene and T-Cell Receptor γ-Chain Gene Rearrangements and Epstein-Barr Virus in ALK+ and ALK− Anaplastic Large.

The Frequency of Immunoglobulin Heavy Chain Gene and T-Cell Receptor γ-Chain Gene Rearrangements and Epstein-Barr Virus in ALK+ and ALK− Anaplastic Large.
Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis  Jeanne A. Jordan, Mary Beth.

Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis  Jeanne A. Jordan, Mary Beth.
Optimization of a Relative Telomere Length Assay by Monochromatic Multiplex Real- Time Quantitative PCR on the LightCycler 480  Anthony Y.Y. Hsieh, Sara.

Optimization of a Relative Telomere Length Assay by Monochromatic Multiplex Real- Time Quantitative PCR on the LightCycler 480  Anthony Y.Y. Hsieh, Sara.
Development and Evaluation of a SYBR Green–Based Real-Time Multiplex RT-PCR Assay for Simultaneous Detection and Serotyping of Dengue and Chikungunya.

Development and Evaluation of a SYBR Green–Based Real-Time Multiplex RT-PCR Assay for Simultaneous Detection and Serotyping of Dengue and Chikungunya.
Establishment and Study of Different Real-Time Polymerase Chain Reaction Assays for the Quantification of Cells with Deletions of Chromosome 7  Elia Mattarucchi,

Establishment and Study of Different Real-Time Polymerase Chain Reaction Assays for the Quantification of Cells with Deletions of Chromosome 7  Elia Mattarucchi,
Development and Evaluation of a Multiplex Real-Time Polymerase Chain Reaction Procedure to Clinically Type Prevalent Salmonella enterica Serovars  Nélida.

Development and Evaluation of a Multiplex Real-Time Polymerase Chain Reaction Procedure to Clinically Type Prevalent Salmonella enterica Serovars  Nélida.
Simultaneous Genotyping of GSTT1 and GSTM1 Null Polymorphisms by Melting Curve Analysis in Presence of SYBR Green I  Fátima Marín, Nadia García, Xavier.

Simultaneous Genotyping of GSTT1 and GSTM1 Null Polymorphisms by Melting Curve Analysis in Presence of SYBR Green I  Fátima Marín, Nadia García, Xavier.
Rapid Assessment of the Heterogeneous Methylation Status of CEBPA in Patients with Acute Myeloid Leukemia by Using High-Resolution Melting Profile  Tsung-Chin.

Rapid Assessment of the Heterogeneous Methylation Status of CEBPA in Patients with Acute Myeloid Leukemia by Using High-Resolution Melting Profile  Tsung-Chin.
B-Cell Clonality Determination Using an Immunoglobulin κ Light Chain Polymerase Chain Reaction Method  Reetesh K. Pai, Artemis E. Chakerian, John M. Binder,

B-Cell Clonality Determination Using an Immunoglobulin κ Light Chain Polymerase Chain Reaction Method  Reetesh K. Pai, Artemis E. Chakerian, John M. Binder,
Homogeneous Polymerase Chain Reaction Nucleobase Quenching Assay to Detect the 1-kbp Deletion in CLN3 That Causes Batten Disease  Paul G. Rothberg, Denia.

Homogeneous Polymerase Chain Reaction Nucleobase Quenching Assay to Detect the 1-kbp Deletion in CLN3 That Causes Batten Disease  Paul G. Rothberg, Denia.

Similar presentations

Ads by Google