1. Fig. 3 Conformational ensembles of apo and ligated FimH variants in solution.
Conformational ensembles of apo and ligated FimH variants in solution. (A) Structural comparison heat map of SAXS profiles indicates varying degrees of conformational similarity among FimGNteH variants, as measured by χ2, ranging from high (blue) to low (red) similarity. (B) Normalized pair distance distributions of FimGNteH variants in the absence or presence of 4Z269. FimGNteH variants are represented as solid lines in the absence of 4Z269 or dotted lines in the presence of 4Z269 and color-coded, as indicated by the colored lines in (A). (C) Structural comparison heat map indicates varying degrees of conformational similarity of each FimGNteH variant in the absence or presence of mannoside 4Z269 at a 2× molar ratio. Color-coded as in (A). (D) Averaged ab initio models of FimGNteH variants in the absence or presence of 4Z269 are color-coded, as previously indicated.
Vasilios Kalas et al. Sci Adv 2017;3:e
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