1. Volume 116, Issue 6, Pages 1358-1366 (June 1999)
Cell cycle block at G1-S or G2-M phase correlates with differentiation of Caco-2 cells: Effect of constitutive insulin-like growth factor II expression 
Raffaele Zarrilli*, Sandro Pignata*,‡, Anna Apicella*,‡, Anna Di Popolo*, Annamaria Memoli*, Paolo Ricchi*,‡, Salvatore Salzano*, Angela Maria Acquaviva* 
Gastroenterology 
Volume 116, Issue 6, Pages (June 1999)
DOI: /S (99)
Copyright © 1999 American Gastroenterological Association Terms and Conditions

2. Fig. 1
Effect of Ara-C treatment on (A) sucrase activity and (B) apo A-I mRNA levels in Caco-2 cells at day 3 of culture. At the end of the incubation time (24 hour), Ara-C–treated cells were directly harvested for enzyme activity or Northern analysis. Northern blots were performed using 10 μg of total RNA per lane, and filters were sequentially hybridized to human apo A-I and human 28S rRNA probes. A representative autoradiograph of 4 separate experiments.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

3. Fig. 2
Effect of nocodazole treatment on (A) sucrase activity and (B) apo A-I mRNA levels in Caco-2 cells at day 3 of culture. After 24-hour incubation with nocodazole at concentrations of 100 and 400 ng/mL (100 N and 400 N, respectively), mitotic-arrested cells were mechanically detached by pipetting. Either plated (P) or detached (D) cells were harvested for enzyme activity or Northern analysis. Northern blotting and filter hybridization were as described in Figure 1. A representative autoradiograph of 4 separate experiments.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

4. Fig. 3
Effect of Ara-C treatment for 48 hours on (A) apo A-I or (B) SI mRNA levels in NEO- or IGF-II–transfected Caco-2 cells at day 3 of culture. Ara-C concentrations used are indicated on the top of the figure. Northern blots were performed using 20 μg of total RNA per lane, and filters were hybridized to the probes shown. Representative autoradiographs.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

5. Fig. 4
Effect of nocodazole treatment for 24 hours on (A) apo A-I and (B) SI mRNA levels in NEO- or IGF-II–transfected Caco-2 cells at day 3 of culture. Nocodazole concentrations are expressed as ng/mL (100 N, 200 N, and 400 N) and are indicated on the top of the figure. Either plated (P) or detached (D) nocodazole-treated cells were harvested for Northern analysis. Isolation of total RNA, Northern blotting, and filter hybridization were as described in Figure 3. A representative autoradiograph of 3 separate experiments.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

6. Fig. 5
Effect of nocodazole on thymidine incorporation in (A) NEO- or (B) IGF-II–transfected Caco-2 cells. Cells were synchronized by double-thymidine block and released into standard culture medium with (▴) or without (●) 0.2 μg/mL nocodazole in the presence of [methyl-3H]thymidine (1 μCi/well). Thymidine incorporation is expressed as counts per minute per 103 cells. Data are representative of 1 out 4 similar experiments in which each point is the mean of triplicate determinations.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

7. Fig. 6
Effect of nocodazole on ploidy in NEO- or IGF-II–transfected Caco-2 cells. Asynchronous cultures of cells were treated with nocodazole at the indicated times, fixed, stained with propidium iodide, and analyzed for DNA content by flow cytometry. (A) Representative contour graphs of untreated and 72-hour nocodazole-treated NEO- and IGF-II–transfected cells. Horizontal axis, fluorescence width; vertical axis, fluorescence area. (B) Line graphs summarizing the percentages of NEO- and IGF-II–transfected Caco-2 cells with more than 4N DNA content over the 72-hour time course for DNA content analysis. Data are representative of 3 similar experiments.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

8. Fig. 7
Effect of Ara-C and nocodazole treatments on p21 and pRb expression in NEO- and IGF-II–transfected Caco-2 cells. Western immunoblot analysis of protein lysates from NEO- and IGF-II–transfected control untreated (C) or 10 μg/mL Ara-C or 200 ng/mL nocodazole (noco)-treated cells using antibodies to pRb and p21. The hyperphosphorylated and hypophosphorylated forms of pRb are indicated (ppRb and pRb, respectively). Data are representative of 3 similar experiments.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions