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Comparison of fluorometric and real-time PCR quantification of DNA extracted from formalin fixed tissue  Jirina Josefiova, Radovan Matura, Jitka Votrubova,

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Presentation on theme: "Comparison of fluorometric and real-time PCR quantification of DNA extracted from formalin fixed tissue  Jirina Josefiova, Radovan Matura, Jitka Votrubova,"— Presentation transcript:

1 Comparison of fluorometric and real-time PCR quantification of DNA extracted from formalin fixed tissue  Jirina Josefiova, Radovan Matura, Jitka Votrubova, Tomas Vojacek, Petr Tomasek, Daniel Vanek  Forensic Science International: Genetics Supplement Series  Volume 6, Pages e137-e139 (December 2017) DOI: /j.fsigss Copyright © 2017 Elsevier B.V. Terms and Conditions

2 Fig. 1 Comparison of qPCR and fluorescent dye-based DNA quantitation for liver tissue of different post-mortem intervals. The X-axis shows the time and method used for fixation: 4% non-buffered formaldehyde (4nBF), 4% buffered formaldehyde (4BF), 36% non-diluted, non-buffered formaldehyde (36nBF). Degraded formaldehyde was simulated by adding 4% formic acid to 4% buffered formaldehyde (4BF+A). Fixation times were 1, 22, and 46h. Forensic Science International: Genetics Supplement Series 2017 6, e137-e139DOI: ( /j.fsigss ) Copyright © 2017 Elsevier B.V. Terms and Conditions


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