1. Volume 115, Issue 1, Pages 157-166 (July 1998)
Role of cytokines in ethanol-induced cytotoxicity in vitro in Hep G2 cells 
Manuela G. Neuman*, Neil H. Shear*, Stefano Bellentani‡, Claudio Tiribelli‡ 
Gastroenterology 
Volume 115, Issue 1, Pages (July 1998)
DOI: /S (98)
Copyright © 1998 American Gastroenterological Association Terms and Conditions

2. Fig. 1
(I) IL-1a, (II) IL-6, and (III) TNF-α expression in cells exposed to 0, 1 × 80, and 2 × 80 mmol/L EtOH. Treatment EtOH I: IL-1α (%) II: IL-6 (%) III: TNF-α (%) Control (0 mmol/L) × 80 mmol/L 142 ± 2a 126 ± 5a 152 ± 4a 2 × 80 mmol/L 160 ± 14a 150 ± 4a,b 170 ± 2a,b aP< vs. control; bP< vs. the first dose of EtOH. Hep G2 cells were seeded in flasks (1 × 106 cells/mL) and exposed to either plain medium (lane A) or 80 mmol/L EtOH for 24 hours (lane B) or to 80 mmol/L EtOH for two 24-hour periods (lane C). Total cellular RNA was isolated from Hep G2 cells. Cytokine gene expression was evaluated by RT-PCR. The amount of DNA was normalized for the amount of G3PDH. Analysis of the expression was performed in triplicate in at least three different cell populations. Data are given as percentages with controls at 100%. For statistical analysis, ANOVA with Bonferroni correction was used.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

3. Fig. 2
Effect of 50 mmmol/L TUDCA or 50 mmmol/L UDCA on EtOH-enhanced cytokine expression. (I) IL-1α, (II) IL-6, and (III) TNF-α. Treatment EtOH I: IL-1α (%) II: IL-6 (%) III: TNF-α (%) EtOH 80 mmol/L ± ± ± 4.0 TUDCA + EtOH ± 5.0a ± 2.0a ± 5.0a UDCA + EtOH ± 2.0a ± 3.0a ± 10.0b aP< 0.05 vs. the dose of EtOH; bP< vs. EtOH (ANOVA with Bonferroni correction). Cells were exposed for 24 hours to 80 mmol/L EtOH (lane A) or to EtOH in the presence of bile acids: 50 μmol/L TUDCA (lane B) or 50 μmol/L UDCA (lane C). RNA was extracted and amplified by RT-PCR. The PCR product was electrophoresed on a 2% agarose gel. The expression of each specific cytokine was quantitated by densitometry and is given as a percentage, with controls at 100%. Data are given as means ± SD.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions