1. Evaluation of a single-tube real-time PCR for detection and identification of 11 dermatophyte species in clinical material 
A.M.C. Bergmans, M. van der Ent, A. Klaassen, N. Böhm, G.I. Andriesse, R.G.F. Wintermans 
Clinical Microbiology and Infection 
Volume 16, Issue 6, Pages (June 2010)
DOI: /j x
Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

2. FIG. 1
Multiple sequence alignments of partial internal transcribed sequence 1 regions from all dermatophyte species detected by the LightCycler real-time PCR assay. Nucleotides corresponding to the species-specific hybridization probe sets are bold and underlined.
Clinical Microbiology and Infection  , DOI: ( /j x)
Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

3. FIG. 2
(a) Typical melting curve analysis result in channel 670 of the LightCycler 2.0, after real-time PCR amplification of Trichophyton interdigitale, Trichophyton mentagrophytes, and Trichophyton tonsurans (DNA from cultured isolates spiked into DNA from negative clinical samples), using the species-specific hybridization probe set Tinmeto1S + Tinmeto3A. The values on the y-axis are the first negative derivative of the change in fluorescence (dFluorescence (670/back530)) divided by the change in temperature (dT). The three vertical lines show the melting temperatures (Tm) of the three dermatophyte species with the probe set. (b) Melting curve analysis result in channel 670 from clinical samples containing T. tonsurans (Tm c. 58°C) or T. interdigitale (Tm c. 65°C).
Clinical Microbiology and Infection  , DOI: ( /j x)
Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions