1. Group Name
MRSA1
Group member name

2. Isolates Sample name Subtype Notes ERR070039-P11 ST2371
From Patient 11
ERR P15
From Patient 15
ERR P26
From Patient 26
ERR HW
From Health Care Worker - misses the ermC plasmid
ERR not-outbreak
ST1
Not part of the outbreak

3. Result of species identification (KmerFinder)
Strain
Species acc. to Harris et al 2013
Predicted species
Closest matching strain
Template coverage
ERR P11
ERR P15
ERR P26
ERR HW
ERR not-outbreak

4. Representative KmerFinder result

5. Result of Multilocus Sequence Typing (MLST)
Strain
ST acc. to Harris et al 2014
Predicted ST
ERR P11
ERR P15
ERR P26
ERR HW
ERR not-outbreak

6. Representative Multilocus Sequence Typing result

7. Result of antibiotics resistance analysis
Isolate
Cefoxitin/Methicillin (MecA)
Cipro-floxacin (chromo-somal mutation, norA)
Erythro-mycin (Erm(A), Erm(B), Erm(C))
Fusidic acid (chromo-somal mutation)
Gentamicin (aac(6’)-aph(2''))
Mupirocin (chromo-somal mutation)
Rifampicin (chromo-somal mutation)
Tetra-cycline (tetC, tetM, tetR, tetS
Vancomycin (Chromo-somal mutations or the vanA or vanB gene cluster.)
Neomycin (aadD, aac(6')-aph(2''))
P11
P15
P26 HW
non-out-break
How is the agreement with the results presented in Harris et al 2013?

8. Result of plasmid analysis
Isolate
rep10
repUS12
rep20
rep5
rep16
rep7
ERR P11
ERR P15
ERR P26
ERR HW
ERR not-outbreak
For each strain write which node the replicon was found on. If not found, leave the cell empty.
Which plasmid is the ermC gene on?

9. Distance matrix made by NDtree and tree made with neighbour-joining

10. Distance matrix made by CSIPhylogeny and tree made with neighbour-joining