1. Effect of motilin and erythromycin on calcium-activated potassium channels in rabbit colonic myocytes 
Gang Lu, Michael G. Sarr, Joseph H. Szurszewski 
Gastroenterology 
Volume 114, Issue 4, Pages (April 1998)
DOI: /S (98)
Copyright © 1998 American Gastroenterological Association Terms and Conditions

2. Fig. 1
Effect of command voltage (Vcom) on single-channel activity of a cell-attached patch. Both the recording pipette and bath solutions contained 150 mmol/L KCl and 5 μmol/L Ca2+ (CRS, Table 1). Thus, membrane voltage was held near 0 mV. Stepping to increasing negative command voltages increased single-channel (A) current amplitude and (A and B) open probability. ▴, CRS (150 mmol/L K+) in pipette and bath. Results are mean ± SEM (n = 5). The dashes to the left in A indicate open state; outward current is upward.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

3. Fig. 1
Effect of command voltage (Vcom) on single-channel activity of a cell-attached patch. Both the recording pipette and bath solutions contained 150 mmol/L KCl and 5 μmol/L Ca2+ (CRS, Table 1). Thus, membrane voltage was held near 0 mV. Stepping to increasing negative command voltages increased single-channel (A) current amplitude and (A and B) open probability. ▴, CRS (150 mmol/L K+) in pipette and bath. Results are mean ± SEM (n = 5). The dashes to the left in A indicate open state; outward current is upward.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

4. Fig. 2
Single-channel activity was recorded in the cell-attached patch configuration. PSS was present in both the pipette and bath. Single-channel activity was first observed when Vcom was −100 mV.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

5. Fig. 3
Effect of TEA and ChTX on single-channel activity of a cell-attached patch. Both the bath and pipette solutions were PSS (Table 1). ■, PSS in pipette and bath (n = 4); ▾, 5 mmol/L TEA in pipette, PSS in bath (n = 7); ★, 125 nmol/L ChTX in pipette, PSS in bath (n = 4). Results are mean ± SEM. *P < 0.05, **P < 0.01 compared with PSS in pipette and bath.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

6. Fig. 4
Effect of the calcium channel agonist BayK 8644 on KCa channel activity in a cell-attached patch. BayK 8644 increased channel activity presumably by increasing cytoplasmic free calcium concentration. ■, PSS in pipette and bath (n = 4); ♦, BayK 8644 (10−6 mol/L) in the bath (n = 6). Results are mean ± SEM. *P < 0.05, **P < 0.01 compared with PSS in pipette and bath.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

7. Fig. 5
(A and B) Single-channel activity of an excised inside-out patch configuration recorded at a Vcom of −20 mV with CRS (Table 1) in the recording pipette and bath. Increasing free Ca2+ concentration to 5 μmol/L (B) increased channel opening. The dashes to the left in B indicate open state; outward current is upward.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

8. Fig. 6
Effect of command voltage on channel activity of an excised inside-out patch using a voltage ramp protocol and symmetrical K+ (150 mmol/L, 150 mmol/L KCl [IPRS], Table 1). (A) Single-channel activity of 10 superimposed voltage ramps. (B) Mean current-voltage relationship obtained from four different patches. The solid line was calculated using the Goldman–Hodgkin–Katz equation.17,18
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

9. Fig. 7
Effect of increasing concentrations of (A) motilin and (B) erythromycin on NPo in the cell-attached patch configuration. PSS (Table 1) was present in the recording pipette and bath. Results are mean ± SEM. *P < 0.05, **P < 0.01 compared with control.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

10. Fig. 7
Effect of increasing concentrations of (A) motilin and (B) erythromycin on NPo in the cell-attached patch configuration. PSS (Table 1) was present in the recording pipette and bath. Results are mean ± SEM. *P < 0.05, **P < 0.01 compared with control.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

11. Fig. 8
Comparison of the effect of (A) motilin and (B) erythromycin on whole-cell current. PSS (Table 1) was present in the bath and WCRS (Table 1) in the recording pipette. Results are mean ± SEM (n = 5 for motilin, n = 6 for erythromycin). *P < 0.05, **P < 0.01, ***P < compared with control. For further details, see text.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

12. Fig. 8
Comparison of the effect of (A) motilin and (B) erythromycin on whole-cell current. PSS (Table 1) was present in the bath and WCRS (Table 1) in the recording pipette. Results are mean ± SEM (n = 5 for motilin, n = 6 for erythromycin). *P < 0.05, **P < 0.01, ***P < compared with control. For further details, see text.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

13. Fig. 9
Effect of motilin and erythromycin on single-channel activity of membrane patches in the cell-attached configuration and of the same membrane patches in the excised patch configuration. In both recording configurations, the agonist was present in the pipette solution and CRS (Table 1) was present in the recording pipette and bath solutions. Note that motilin and erythromycin increased NPo of the cell-attached patches but had no effect on the same patches excised in the inside-out configuration. Control NPo values of excised inside-out patches were obtained from four other patches. Results are mean ± SEM (n = 4). *P < 0.05 compared with cell attached.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions