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Efficient gene transfer method into the whole heart through the coronary artery with hemagglutinating virus of Japan liposome  Yoshiki Sawa, MDa, Keishi.

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Presentation on theme: "Efficient gene transfer method into the whole heart through the coronary artery with hemagglutinating virus of Japan liposome  Yoshiki Sawa, MDa, Keishi."— Presentation transcript:

1 Efficient gene transfer method into the whole heart through the coronary artery with hemagglutinating virus of Japan liposome  Yoshiki Sawa, MDa, Keishi Kadoba, MDa, Ken Suzuki, MDa, Hong-Zhi Bai, MDa, Yasufumi Kaneda, MDb, Ryota Shirakura, MDc, Hikaru Matsuda, MDa  The Journal of Thoracic and Cardiovascular Surgery  Volume 113, Issue 3, Pages (March 1997) DOI: /S (97) Copyright © 1997 Mosby, Inc. Terms and Conditions

2 Fig. 1 Localization of FITC-ODN transfection. The H group (A) resulted in an efficient and diffuse transfer of FITC-ODN into nuclei of the myocytes in the myocardium. Transfer was less efficient and diffuse in group L (B). The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

3 Fig. 1 Localization of FITC-ODN transfection. The H group (A) resulted in an efficient and diffuse transfer of FITC-ODN into nuclei of the myocytes in the myocardium. Transfer was less efficient and diffuse in group L (B). The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

4 Fig. 2 Efficiency of FITC-ODN transfection. A, Percentage of FITC-stained nuclei of myocytes in the H group (75% ± 14%) was significantly higher than that in the L group (7% ± 5%). B, The H group (979 ± 1128 FI/gm) showed significantly higher intensity of FITC (FI/gm ventricular wet weight) than did the L group (116 ± 68 FI/gm). The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

5 Fig. 3 Efficiency of transfection of β-galactosidase The hearts in the H group (A, original magnification ×80) showed apparent histochemical expression of β-galactosidase on the myocytes. However, no such expression of β-galactosidase was seen in group L (B, original magnification ×80). The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

6 Fig. 3 Efficiency of transfection of β-galactosidase The hearts in the H group (A, original magnification ×80) showed apparent histochemical expression of β-galactosidase on the myocytes. However, no such expression of β-galactosidase was seen in group L (B, original magnification ×80). The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

7 Fig. 4 Western blotting analysis of Mn-SOD. Western blotting analysis showed an apparent increased expression of Mn-SOD at 25 kd in the Mn-SOD transfected hearts as compared with the control hearts. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

8 Fig. 5 The efficacy of the gene transfection of Mn-SOD. The percentage recoveries of control value of left ventricular end-diastolic pressure (LVDP; S vs C, 86% ± 3% vs 54% ± 12%) and coronary flow (98% ± 2% vs 66% ± 12% ) were significantly higher in the Mn-SOD transfected group (S) than in the control group (C). The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

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