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Ruben J. Geerdink, MSc, Marije P. Hennus, PhD, MD, Geertje H. A

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Presentation on theme: "Ruben J. Geerdink, MSc, Marije P. Hennus, PhD, MD, Geertje H. A"— Presentation transcript:

1 LAIR-1 limits neutrophil extracellular trap formation in viral bronchiolitis 
Ruben J. Geerdink, MSc, Marije P. Hennus, PhD, MD, Geertje H.A. Westerlaken, BSc, Alferso C. Abrahams, MD, Kim I. Albers, MD, Jona Walk, MD, Esther Wesselink, MD, Riny Janssen, PhD, Louis Bont, PhD, MD, Linde Meyaard, PhD  Journal of Allergy and Clinical Immunology  Volume 141, Issue 2, Pages (February 2018) DOI: /j.jaci Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 In vivo activated human neutrophils express LAIR-1. Representative example of the gating strategy for neutrophils from blood and BAL of patients with RSV bronchiolitis (n = 9) (A), the quantified expression of inhibitory receptors as MFI (n = 9) (B), and a representative example of activation markers (C) on these neutrophils (n = 9). D, LAIR-1 expression on peripheral blood neutrophils of patients with sepsis (n = 4) and a representative healthy control child. E, Surface expression of LAIR-1 after in vitro stimulation (n = 3). F, Representative image of intracellular LAIR-1 expression by resting neutrophils (n = 3). BAL, Bronchoalveolar lavage; cIg, isotype-matched control antibody; fMLF, formyl-methionyl-leucyl phenylalanine; FSC, forward scatter; LAIR-1, leukocyte-associated immunoglobulin-like receptor-1; MFI, median fluorescence intensity; PAF, platelet-activating factor; Siglec-9, sialic acid-binding immunoglobulin-like lectin-9; SIRPα, signal-regulatory proteinα; SSC, side scatter. ***P < .001 and ****P < Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 LAIR-1 suppresses NET formation by airway-infiltrated neutrophils obtained from patients with RSV bronchiolitis. A, Representative example of SYTOX Green-visualized NETs produced by neutrophils from blood and BAL of patients with RSV bronchiolitis (n = 6) incubated in vitro with an isotype-matched control antibody (cIg), anti–SIRL-1 antibody, anti–LAIR-1 antibody, or medium only. B, Quantified NET area normalized to medium only for each patient (n = 6). C, Costaining of Hoechst and SYTOX Green-positive NET DNA with MPO (n = 6). D, Representative example of surface expression of LAIR-1 and SIRL-1 on neutrophils from blood and BAL of patients with RSV bronchiolitis (n = 9). BAL, Bronchoalveolar lavage; cIg, isotype-matched control antibody; LAIR-1, leukocyte-associated immunoglobulin-like receptor-1; MFI, median fluorescence intensity; MPO, myeloperoxidase; SIRL-1, signal inhibitory receptor on leukocytes-1. *P < .05. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig E1 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig E2 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig E3 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig E4 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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