1. STAT3 regulates IgE class switching in a Th2-type response.
STAT3 regulates IgE class switching in a Th2-type response. STAT3f/f control or CD2-Cre; STAT3f/f mice were immunized i.p. with 100 μg OVA in Alum. (A) Sera were collected at indicated time points and OVA Ab levels of different isotypes (IgE, IgG, and IgG1) were detected by ELISA. Data are means ± SEM. (B and C) FACS analysis of splenocytes from day 14 postimmunization mice. Cells were stained with mAbs B220, GL7, CD138, IgG1, and intracellular IgE. Cells were gated based on the corresponding isotype control mAb staining. Representative dot plots and summarized results are shown from at least three independent experiments. Data are means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < , unpaired two-tailed Student t test).
Paul Dascani et al. ImmunoHorizons 2018;2:
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