1. Experimental timeline.
Experimental timeline. Thirty-two mice were randomly divided into BICAO and Sham operation groups. The behavioural tests of sucrose preference, tail suspension, forced swim and pole tests started at days 7, 21 and 30 after Sham or BICAO treatment (n=10 per group). After the last behavioural experiment, 20 mice (n=10 per group) were sacrificed and mouse brains were processed for immunofluorescent staining of neuronal marker neuronal nuclei (NeuN). For western blot experiments, 12 mice (n=6 per group) were sacrificed 1 day after Sham or BICAO treatment. BICAO, bilateral internal carotid artery occlusion; FST: forced swim test; IF, immunofluorescence; PT: pole test; SPT, sucrose preference test; TST, tail suspension test.
Shuiqiao Liu et al. Stroke Vasc Neurol 2017;svn
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