1. Fig. 1. mGlu7 expression is reduced in RTT autopsy samples.
mGlu7 expression is reduced in RTT autopsy samples. (A) Diagram of luciferase reporter constructs. (B) Bar graph showing the effect of transfection of increasing amounts of human MECP2 plasmid on luciferase expression from the pGL4 vector with and without the GRM7 promoter [#P < and *P < , one-way analysis of variance (ANOVA) with Bonferroni’s post hoc; n = 3]. (C) GRM7 messenger RNA (mRNA) expression in the motor cortex of control and RTT autopsy samples (P = 0.07, two-tailed Student’s t test; n = 7 to 8 biological replicates, df = 13). (D) mGlu7 (***P < , two-tailed Student’s t test; n = 7 to 8, df = 13) and (E) vGlut2 (P = 0.39, two-tailed Student’s t test; n = 7 to 8, df = 13) protein expression in control and RTT autopsy samples. (F) Representative Western blots showing mGlu7 and vGlut2 expression in control and RTT motor cortex samples. MECP2 mutations are listed below the representative images.
Rocco G. Gogliotti et al., Sci Transl Med 2017;9:eaai7459
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