1. Quantitative and Qualitative Analyses of the SNRPN Gene Using Real-Time PCR with Melting Curve Analysis 
Chia-Cheng Hung, Shin-Yu Lin, Shuan-Pei Lin, Chih-Ping Chen, Lang-Yao Chen, Chien-Nan Lee, Yi-Ning Su 
The Journal of Molecular Diagnostics 
Volume 13, Issue 6, Pages (November 2011)
DOI: /j.jmoldx
Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2. Figure 1
Amplification curves of SYBR Green I-based real-time PCR analysis for detection of gene copy numbers of the SNRPN gene. The settings of the real-time PCR are the cycle range set between 22 and 34 cycles, the noise band set at , and the threshold of estimation of the threshold cycle value was The results are shown by green (unaffected individuals), pink [Prader-Willi syndrome (PWS) with uniparental disomy (UPD) type], red (PWS with deletion type), and gray [Angelman syndrome (AS) with deletion type].
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3. Figure 2
The scatter plots of the calculated copy numbers by SYBR green-I–based real-time PCR are illustrated for the wild type, Prader-Willi syndrome (PWS) with deletion, PWS with uniparental disomy, and Angelman syndrome (AS) with deletion. UPD, uniparental disomy.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

4. Figure 3
A: Normalized melting curve analysis of the SNRPN gene, and (B) the derivative plot [negative derivative of the fluorescence (F) with respect to temperature (T) versus temperature] of the SNRPN gene. The melting curves in blue are Prader-Willi syndrome (PWS) samples, the melting curves in red are wild types, and the melting curves in green are Angelman syndrome samples. The unmethylated melting peak was at 85°C, and the methylated melting peak was at 89°C. The results are shown by green (unaffected individuals), pink [PWS with uniparental disomy (UPD) type], red (PWS with deletion type), and gray [Angelman syndrome (AS) with deletion type].
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions