1. Candidates with a PVM localization.
Candidates with a PVM localization. Live-cell microscopy images of developing blood stage merozoites (upper panels) or free blood stage merozoites (middle panels) as well as an IFA of fixed liver stage cytomeres stained with an antiserum against EXP1 (lower panels) are shown for parasites expressing endogenously GFP-tagged PBANKA_ (A), PBANKA_ (B), or PBANKA_ (C). The endogenously GFP-tagged proteins are shown in green, the cytosolic mCherry proteins are shown in red, and the EXP1 signal in the IFA of fixed liver stage parasites is displayed in purple. DNA of Hoechst- or DAPI-stained nuclei is shown in blue. DIC, differential interference contrast. Scale bars for all blood stages; 2 μm; scale bars for the liver stage, 10 μm.
Cilly Bernardette Schnider et al. mSphere 2018; doi: /mSphere