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Novel regenerative therapy using cell-sheet covered with omentum flap delivers a huge number of cells in a porcine myocardial infarction model  Yasuhiro.

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Presentation on theme: "Novel regenerative therapy using cell-sheet covered with omentum flap delivers a huge number of cells in a porcine myocardial infarction model  Yasuhiro."— Presentation transcript:

1 Novel regenerative therapy using cell-sheet covered with omentum flap delivers a huge number of cells in a porcine myocardial infarction model  Yasuhiro Shudo, MD, Shigeru Miyagawa, MD, PhD, Satsuki Fukushima, MD, PhD, Atsuhiro Saito, PhD, Tatsuya Shimizu, MD, PhD, Teruo Okano, PhD, Yoshiki Sawa, MD, PhD  The Journal of Thoracic and Cardiovascular Surgery  Volume 142, Issue 5, Pages (November 2011) DOI: /j.jtcvs Copyright © Terms and Conditions

2 Figure 1 Study protocol for the assessment of cardiac function and histologic analysis. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © Terms and Conditions

3 Figure 2 Macroscopic (×40) views of the heart in the 4 groups (hematoxylin–eosin staining). SO group, skeletal myoblast (SMB) sheets wrapped with omentum; S group, SMB sheets only; O group, omentum only; sham group, sham operation. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © Terms and Conditions

4 Figure 3 Macroscopic (×40) views of the heart in the 4 groups (Masson trichrome staining). Infarct area: The SO group showed a significant improvement in the infarct area of the whole heart. Abbreviations as in Figure 2. ∗P < .05. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © Terms and Conditions

5 Figure 4 Microscopic (×200) views of sections of the peri-infarct border-zone region stained with the von Willebrand factor antibody (factor VIII) in the 4 groups (bar = 20 μm). Vascular density: The SO group showed a significant improvement in vascular density, as assessed by anti–von Willebrand factor antibody. Abbreviations as Figure 2. N > 4 in each group. ∗P < .05. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © Terms and Conditions

6 Figure 5 A, Microscopic (×200) views of the infarct region in the SO group (alpha smooth muscle actin staining). B, Microscopic (×200) views of the infarct region in the SO group (vimentin staining). C, Microscopic (×200) views of the infarct region in the SO group (fast type myosin heavy chain staining). D and E, Microscopic (×200) views of the heart (desmin staining). SO group, SMB sheets wrapped with omentum. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © Terms and Conditions

7 Figure 6 mRNA expressions in implanted infarcted hearts were determined by real-time PCR using porcine primers. The expressions of vascular endothelial growth factor (VEGF) and signal transducer and activator of transcription 3 (STAT3) mRNA were significantly increased in the SO group compared with the other groups. Abbreviations as in Figure 2. N > 4 in each group. ∗P < .05. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © Terms and Conditions

8 Figure 7 Echocardiographic analysis. The SO group showed less ventricular remodeling and greater degrees of improved anterior wall thickness and cardiac function than did the S group. Pink line, SO group; red line, S group; blue line, O group; green line, sham group; LVEDV, left ventricular end-diastolic volume; LVESV, left ventricular end-systolic volume; LVEF, left ventricular ejection fraction; other abbreviations as in Figure 2. N = 6 in each group. ∗P < .05. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © Terms and Conditions

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