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Sensor optimization for thiosulfate and tetrathionate detection in the gut Sensor optimization for thiosulfate and tetrathionate detection in the gut A–F(A.

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Presentation on theme: "Sensor optimization for thiosulfate and tetrathionate detection in the gut Sensor optimization for thiosulfate and tetrathionate detection in the gut A–F(A."— Presentation transcript:

1 Sensor optimization for thiosulfate and tetrathionate detection in the gut
Sensor optimization for thiosulfate and tetrathionate detection in the gut A–F(A and D) Plasmid design of the constitutive sensors in Escherichia coli Nissle (B and E) Comparison of the inducible sensors in BW28357, the constitutive sensors in Nissle 1917, and D‐to‐A inactivated sensors for thiosulfate (B) and tetrathionate (E). GFP output is shown in the absence (white bars) and presence of 1 mM tetrathionate or 5 mM thiosulfate (black bars), respectively. (C and F) Normalized dose–response relationship of thiosulfate (C) and tetrathionate sensors (F). Shown is the original inducible BW28357 strain grown aerobically (closed circles, red curve fit), and a constitutive promoter strain in Nissle grown aerobically (closed squares) or anaerobically (open squares). Different constitutive promoters were used for the aerobic and anaerobic Nissle strains to achieve the best dynamic range. A shift in half‐maximal response indicates sensitivity to oxygen.Data information: Data are mean of at least three biological replicates ± SD. Kristina N‐M Daeffler et al. Mol Syst Biol 2017;13:923 © as stated in the article, figure or figure legend

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