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Published byVeikko Parviainen Modified over 5 years ago
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Ischemic preconditioning reduces neurologic injury in a rat model of spinal cord ischemia
David A. Zvara, MD, David M. Colonna, MD, Dwight D. Deal, BS, Jason C. Vernon, BS, Mamatha Gowda, BS, John C. Lundell, MD The Annals of Thoracic Surgery Volume 68, Issue 3, Pages (September 1999) DOI: /S (99)
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Fig 1 Heart rate, mean proximal aortic pressure, and mean distal aortic pressure during experimental protocol for control group (CTRL) and ischemic preconditioning group (IPC). (BL = baseline; IPC = 3-minute period of aortic occlusion, or ischemic preconditioning; ISC = 12-minute prolonged ischemic insult; REP-1 = 30-minute reperfusion interval; REP-2 = period of reperfusion after ISC [closure of surgical wounds and recovery of animal]; — = interval of significant difference between groups.) The Annals of Thoracic Surgery , DOI: ( /S (99) )
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Fig 2 Data from the four motor neurologic tests (Tarlov scale, righting, placing, and inclined plane) were combined for a composite neurologic score and are presented as a box plot. The scores are shown in Table 1 and are summated for each animal. A score of 10 indicates complete motor neurologic deficit, whereas a 0 indicates a normal animal. Mann-Whitney rank sum test demonstrated significant difference between groups at both time points. (CTRL = control group; IPC = ischemic preconditioning group; boxed area = 25th to 75th percentile; — = 10th and 90th percentiles; ○ = individual animals above or below 10th and 90th percentiles; broken line = median value for group.). The Annals of Thoracic Surgery , DOI: ( /S (99) )
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Fig 3 Photomicrograph of ventral horn of lumbar spinal cord (L5) from rat in ischemic preconditioning group with motor deficit score of 0 48 hours after injury. The gray matter architecture is clearly conserved and shows healthy surviving motor neurons (hatched arrowheads) and relatively low numbers of infiltrates (black arrowheads) in response to the limited damage. The few atrophied and darkly stained neurons (white arrowheads) are an artifact of formalin fixation. Laminae 6 through 10 are numbered and outlined according to Rexed’s criteria [21]. (Hematoxylin and eosin; ×10 before % reduction.) The Annals of Thoracic Surgery , DOI: ( /S (99) )
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Fig 4 Photomicrograph of ventral horn of lumbar spinal cord (L5) from control rat with motor deficit score of hours after aortic occlusion. The process of necrosis is evidenced by the shrunken, anuclear, darkly stained acidophilic motor neurons (hatched arrowheads) and the neuronal phagia (lesions characterized by a remnant empty space left by phagocytized neurons) (black arrowhead). Also typical of necrosis is the marked increase in basophilic infiltrates, which densely surround the microvasculature and diffusely occur around necrotic tissue (white arrowheads). Laminae 6 through 10 are numbered and outlined according to Rexed’s criteria [21]. (Hematoxylin and eosin; ×10 before % reduction.) The Annals of Thoracic Surgery , DOI: ( /S (99) )
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