1. Schematic of electrophoresis and imaging components.
Schematic of electrophoresis and imaging components. Schematic of the electrophoresis system used for active clearing of tissue. A, A recirculating water bath filled with clearing solution is connected via chemical- and heat-resistant tubing to a filter unit (which removes particulate matter and reduces bubbles in the clearing solution). The clearing solution is then split into six parallel tube lines, each containing one ETC chamber. The fluid output of the chambers is then recombined into a single line before entering the recirculator. Power supplies provide current to each ETC chamber. Schematic of the LaVision Biotec UltraMicroscope (B) compared with confocal microscope (C). The light sheet design permits high-speed image capture of large tissue volumes. In contrast, a confocal microscope may be used for high-resolution imaging of CLARITY tissue, although the acquisition rate is much slower.
Jonathan R. Epp et al. eneuro 2015;2:ENEURO
©2015 by Society for Neuroscience