1. Immunoblot analysis of salivary allergens in 10 mosquito species with worldwide distribution and the human IgE responses to these allergens 
Zhikang Peng, MDa,b, Hongbing Li, RTa, F.Estelle R. Simons, MD a,b 
Journal of Allergy and Clinical Immunology 
Volume 101, Issue 4, Pages (April 1998)
DOI: /S (98)
Copyright © 1998 Mosby, Inc. Terms and Conditions

2. FIG. 1
Maps showing worldwide distribution of Ae. aegypti,31 Ae. vexans,32 and Cx. quinquefasciatus.33
Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) )
Copyright © 1998 Mosby, Inc. Terms and Conditions

3. FIG. 2
Salivary allergens of 10 mosquito species. Proteins in saliva or salivary gland extracts of 10 species were separated by SDS-PAGE and then transferred to nitrocellulose membranes. Membranes were sequentially incubated with pooled serum from subjects allergic to mosquitos, monoclonal anti-human IgE, and enzyme-conjugated goat anti-mouse IgG as described in Methods section. Aed a 1 (68 kd) and Aed a 2 (37 kd) of Ae. aegypti are indicated by arrows a and b, respectively.
Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) )
Copyright © 1998 Mosby, Inc. Terms and Conditions

4. FIG. 3
IgE responses to salivary allergens of six mosquito species in subjects allergic to mosquitos and nonallergic subjects. Proteins of saliva or salivary gland extracts separated by SDS-PAGE were transferred to nitrocellulose membranes. Membranes were sequentially incubated with sera from subjects allergic to mosquitos (strips 1 to 12) or sera from nonallergic subjects (strips 13 to 17), monoclonal anti-human IgE, and enzyme-conjugated goat anti-mouse IgG as described in Methods section. Infant serum (strip 18), cord serum (strip 19) , and PBST (strip 20) were used as negative controls. Six mosquito species used were Ae. aegypti (A), Ae. vexans (B), Ae. albopictus (C), Ae. togoi (D), Cx. quinquefasciatus (E), and Cx. pipiens (F). In Ae. vexans a 68 kd species-shared allergen, Aed a 1, is indicated by arrow a, and a 23 kd Ae. vexans–specific allergen is indicated by arrow b.
Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) )
Copyright © 1998 Mosby, Inc. Terms and Conditions

5. FIG. 4
Analysis of shared Aed a 1 and Aed a 2 allergens in different mosquito species. Proteins in four mosquito saliva or salivary gland extracts were separated by SDS-PAGE and then transferred to nitrocellulose membranes. Membranes were incubated with rabbit anti-rAed a 1 (left) or rabbit anti-rAed a 2 (right) followed by incubation with enzyme-conjugated goat anti-rabbit IgG as described in Methods section.
Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) )
Copyright © 1998 Mosby, Inc. Terms and Conditions