1. Kinesin-5 nano-motors in cell division Leah Gheber Departments of Clinical Biochemistry and Chemistry Ilse Katz Institute for Nanoscale Science and Technology Ben-Gurion University of the Negev, Beersheba, Israel

2. Mitotic spindle human cells – specific kinesin-5 inhibitor monastrol
control
5 m
Leizerman et al., CMLS 2005 (Gheber lab)

3. Kinesin-5 motor proteins+ + -
Spindle assembly
Maintenance of bipolar
spindle structure
Anaphase spindle
elongation

4. Our Goal
To understand the mechanisms and regulation of the mitotic kinesin-5 nano motors
Our Strategy
To correlate between in vivo functions and in vitro motor activity of specific kinesin-5 mutants and variants

5. In vivo assays – protein expression and quantitative live imaging
Cin8
cin8-5A
Cin8-3GFP
Cin8
Cin8-5A-3GFP
Cin8-3A
Microtubule plus-end tracking
Tubulin
Kinesin-5

6. In vitro - Single molecule fluorescence motility assay - Kinesin-5-3GFP

7. People who do the work Collaborators Vladimir Fridman
Adina Gerson-Gurwitz Natalia Movshovich
Maria Podolskaya
Rachel Abunie-Masala
Ofer Shapira
Nurit Schkolnik
Alina Goldstein
Adina
Vladimir
Collaborators
Christoph Schmidt – GAUG
Stefan Lakamper – GAUG
Mart Loog – Tartu, Estonia
Liam Holt – UC Berkeley, US
Andy Hoyt – JHU, US
Dina Raveh – BGU, Israel
Rachel
Nurit
Natalia
Alina
Ofer