1. Effector T cells (Th-) Regulatory T cells Treg Th17 Th1 Th2
Supplemental Diglta Content 1 _ Figure 1
Effector T cells (Th-) a/
Regulatory T cells
Treg
Th17
Th1
Th2
FOXP3
RORgt
Tbet
GATA3 * ns ns ns ns ns
***
***
*** **
***
***
TGFb
IL17A
IFNg
IL4 ** ns ns ns
***
*** ns
***
***
*** ns
***
IL10
IL21
TIM3 * ns *
***
*** *
***
***
***

2. ns MEDIUM MPA (3µg/mL) TACROLIMUS (5µg/L) EVEROLIMUS (5µg/L) b/
Supplemental Digital Content 1 – Figure 1 b/ ns
MEDIUM
MPA
(3µg/mL)
TACROLIMUS
(5µg/L)
EVEROLIMUS
(5µg/L)

3. Supplemental Digital Content 1 - Figure 1: Differential effects of MPA, tacrolimus and everolimus upon human CD4+ T cells in vitro polarization and IL17 production.
a/ Effects of MPA, tacrolimus and everolimus upon the transcription of CD4+ cell subsets-related genes after two days of T cell activation. CD4+ T cells were activated following the same protocol, alone or in the presence of MPA and tacrolimus at a final concentration of 3µg/mL and 5µg/L, respectively. A comparative RT-PCR of the relative expression of CD4+ T cell subsets-related genes was then realized.
*, P<0.05; **, P <0.01; ***, P <0.005 (Comparison of means, Wilcoxon signed-rank test, .Prism 5; Graph Pad Softwares, San Diego, Calif). Bars represent medians.
b/ Effects of MPA, tacrolimus and everolimus upon IL17 secretion. CD4+ T cells from 18 healthy volunteers were activated for 48 hours alone or in the presence of MPA and tacrolimus at a final concentration of 3µg/mL and 5µg/L. Frequency of IL17-producing CD4+ T cells among activated CD4+ T cells was measured by ELISpot. The figure presents the comparison of the frequency of IL17A.