1. CD160−/− mice have impaired clearance of oral L
CD160−/− mice have impaired clearance of oral L. monocytogenes infection.
CD160−/− mice have impaired clearance of oral L. monocytogenes infection. (A) Age-matched WT (blue circles, n = 7–16) and CD160−/− (red circles, n = 7–16) littermates were infected with 2 × 109L. monocytogenes orally, and bacterial burden from spleen, mLN, small intestine, and colon was assessed on day 3 postinfection. (B) Bacterial burden from small intestine and colon on day 7 after oral L. monocytogenes infection with 2 × 109L. monocytogenes from age-matched WT (blue circles, n = 14–17) and CD160−/− (red circles, n = 14–17) littermates. (C) Percentages of WT (n = 14–17) and CD160−/− (n = 14–17) littermates that cleared (cyan) versus those with detectable L. monocytogenes (green) in the small intestine and colon on day 7 after L. monocytogenes oral infection with 2 × 109L. monocytogenes. (D) Bacterial burden from spleen and mLN on day 7 after oral L. monocytogenes infection with 2 × 109L. monocytogenes from age-matched WT (blue circles, n = 10) and CD160−/− (red circles, n = 10) littermates. Age-matched RAG−/− (blue circles, n = 5–18) and CD160−/− RAG−/− (red circles, n = 7–18) littermates were infected with 2 × 109L. monocytogenes orally, and bacterial burden from spleen, mLN, and colon was assessed on (E) day 3 and (F) day 7 postinfection. Data are pooled from four independent experiments, (A), (C), and (D). Data are pooled from three independent experiments, (B), (E), and (F). Significance was assessed using Mann–Whitney nonparametric test in (A), (B), (D), and (E) and χ2 test in (C). Data are represented means ± SEM. **p < 0.01.
Catherine L. Tan et al. ImmunoHorizons 2018;2:
Copyright © 2018 The Authors