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Supplementary Figure 1. A

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1 Supplementary Figure 1. A
Supplementary Figure 1. A. Immnocytochemistry of T24 cells transfected with siR-socs7 alone. Bar=20μm. B. The expression levels of STAT3 and p-STAT3 by western blotting analysis in T24 and NKB1 cells transfected with siR-socs7 alone. siRNA was transfected into cells at 20 nM. The assays were performed at 72 (T24) or 96 (NKB1) hr after the transfection. A B T24 NKB1 T24 −PIC −PIC siR-socs7 alone cont siR-socs7 cont siR-socs7 STAT3 p-STAT3 β-actin STAT3 Phalloidin Merge (siRNA; 20 nM)

2 Supplementary Figure 2. A
Supplementary Figure 2. A. Expression levels of IFN-β and ISGs in human melanoma A2058 cells, as determined by real time PCR. B. Protein expression levels in A2058 cells. C. Expression levels of IFN-β, ISGs, and SOCS7 in A2058 cells, as determined by real time PCR. A ** ** ** ** ** ** IFN-β OAS1 TRAIL * cont miR-145 β-actin PARP-1 Caspase-8 −Proform −Cleaved form −Active form; 43 kDa −Active form; 18 kDa B C N.S * * IFN-β OAS1 TRAIL SOCS7


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