1. The enzyme phosphatase
Paul Beaumont & Trisha Geraghty

2. Phosphatase
Phosphatase enzymes from plants: a versatile resource for post-16 courses
Meatyard, B. (1999),
J. Biol. Educ., 33(2),

3. The Assay

4. PP = phenolphthalein (product)
PPP = phenolphthalein phosphate (substrate)

5. Preparing the enzyme extract (Part A)
Running the assay (Part B)

7. Creating the extract

8. Germination

9. Maceration

10. Loading centrifuge tube

11. Centrifugation

12. Retain supernatant

13. Running the Assay

14. Running the assay

15. Investigations Effect of temperature Effect of pH
Phosphatase levels from different species
Phosphatase levels in different locations
Effect of phosphate concentration

16. Results