1. Hydroxypropyl cellulose as an option for supplementation of cryoprotectant solutions for embryo vitrification in human assisted reproductive technologies 
Chiemi Mori, Akiko Yabuuchi, Kenji Ezoe, Nana Murata, Yuko Takayama, Tadashi Okimura, Kazuo Uchiyama, Kei Takakura, Hiroyuki Abe, Keiko Wada, Takashi Okuno, Tamotsu Kobayashi, Keiichi Kato 
Reproductive BioMedicine Online 
Volume 30, Issue 6, Pages (June 2015)
DOI: /j.rbmo
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2. Figure 1
Survival rates of mouse blastocysts after vitrification and warming. Hybrid zona pellucida intact: zona pellucida-intact blastocysts from hybrid mouse strain (C57BL/6JxDBA); inbred zona pellucida-intact: zona pellucida-intact blastocysts from inbred mouse strain (C57BL/6J); inbred zona pellucida-free: zona pellucida-free blastocysts from inbred mouse strain (C57BL/6J). Different letters within each group indicate significant differences (P < 0.05). HPC = hydroxypropyl cellulose; SSS = serum substitute supplement; ZP = zona pellucida.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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3. Figure 2
Survival rates of human blastocysts after re-vitrification and warming; 5% hydroxypropyl cellulose (HPC): 5% HPC-supplemented vitrification media, 5% serum substitute supplement (SSS): 5% SSS-supplemented vitrification media; 20% SSS: 20% SSS-supplemented vitrification media; ZP-intact: ZP-intact blastocysts; ZP-free: ZP-free blastocysts. ZP = zona pellucdia. Different letters within each group indicate significant differences (P < 0.05).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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4. Figure 3
Survival rates of mouse vitrified and warmed blastocysts using vitrification media supplemented with or without hydroxypropyl cellulose (HPC): (A) combinations of HPC-supplemented solutions used; DS = diluent solution; HPC = with HPC; – = without HPC; ES = equilibrium solution; VS = vitrification solution; WS = washing solution; (B) survival rate of blastocysts after vitrification and warming. Different letters indicate significant differences (P < 0.05).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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5. Figure 4
Viscosity of vitrification solution (VS); 1% hydroxypropyl cellulose (HPC): 1% HPC-supplemented VS; 5% HPC: 5% HPC-supplemented VS; 5% serum substitute supplement; (SSS): 5% SSS-supplemented VS; 20% SSS: 20% SSS-supplemented VS. Different letters indicate significant differences (P < 0.05).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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6. Figure 5
Oxygen consumption rates of blastocysts after vitrification and warming; (A) oxygen consumption rates of mouse blastocysts after vitrification and warming; non-vitrified: non-vitrified blastocysts; 5% HPC: 5% HPC-supplemented vitrification media; (B) oxygen consumption rates of human blastocysts after re-vitrification and warming. Non-re-vitrified: non-re-vitrified blastocysts; 5% HPC: 5% HPC-supplemented vitrification media. No difference was found in either group. HPC = hydroxypropyl cellulose.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions