1. Comet assay on mouse oocytes: an improved technique to evaluate genotoxic risk on female germ cells 
Anais Berthelot-Ricou, M.D., Jeanne Perrin, M.D., Ph.D., Carole Di Giorgio, Ph.D., Michel De Meo, Ph.D., Alain Botta, M.D., Ph.D., Blandine Courbiere, M.D., Ph.D. 
Fertility and Sterility 
Volume 95, Issue 4, Pages (March 2011)
DOI: /j.fertnstert
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Examples images of comet assay on mouse oocytes with and without ZP. (A) Mouse oocyte with ZP; negative control, no DNA migration. (B) Mouse oocyte without ZP; negative control, no DNA migration. (C) Mouse oocyte with ZP exposed in vitro to SSI at 12 J/cm2 for 4 minutes: DNA migration. (D) Mouse oocyte without ZP exposed in vitro to SSI at 12 J/cm2 for 4 minutes: DNA migration. The direction of electrophoresis was from left to right, and the comet tail containing the DNA fragments was stained by propidium iodide 0.1 mg/mL. Scale bar, 100 μm; magnification, ×200.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Examples of different aspects of DNA damage as detected by the comet assay on mouse oocytes. (A) Control group: intact oocyte after comet assay process. (B) Oocyte exposed in vitro to SSI (12 J/cm2) for 4 minutes: moderate DNA damage. (C) Oocyte exposed in vitro for 5 minutes to H2O2 (250 μM): major DNA damage. (D) Oocyte exposed in vitro for 1 hour to methylmethanesulfonate (100 μM). Maximal DNA damage, with the aspect called ghost cell. The direction of electrophoresis was from left to right, and the comet tail containing the DNA fragments was stained by iodide propidium 0.1 mg/mL. Scale bar, 100 μm. Magnification, ×200.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Calculated OTMχ2 of various samples. Cont, control; SSI, exposure to simulated sunlight irradiation (12 J/cm2); MMS, exposure to methylmethanesulfonate (50 μM); H2O2, exposure to hydrogen peroxide (250 μM); Dotted line, statistical significance (P<0.001).
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions