1. Volume 147, Issue 5, Pages 1012-1020.e4 (November 2014)
Confocal Endomicroscopy Shows Food-Associated Changes in the Intestinal Mucosa of Patients With Irritable Bowel Syndrome 
Annette Fritscher-Ravens, Detlef Schuppan, Mark Ellrichmann, Stefan Schoch, Christoph Röcken, Jochen Brasch, Johannes Bethge, Martina Böttner, Julius Klose, Peter J. Milla 
Gastroenterology 
Volume 147, Issue 5, Pages e4 (November 2014)
DOI: /j.gastro
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2. Figure 1
Lymphocytes visualized by (A) CLE and (B) immunocytochemistry against CD3.
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3. Figure 2
CLE images of (A) baseline and (B and C) after food challenge findings. (A) Confocal image at baseline shows closely attached villi and vascularity, representing the deepest level of mucosal imaging with CLE. (B) Confocal image after mucosal reaction to food. Multiple eruptions represent breaks in the wall (white arrows), through which fluorescein is secreted into the lumen. The IVS widened and is turning grey instead of the initial black. (C) End stage of the reaction. Because of an influx of fluorescein the IVS turned white and widened further.
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4. Figure 3
Images of a single cell after food challenge shows the onset of mucosal damage. (A–C) The intact epithelium (A) starts to break its continuity (B), forming a leak with eruption of fluorescein into the gut lumen (C). Although a physiological process, the onset of large numbers of breaks represents pathology (Figure 2B).
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5. Figure 4
Comparison of epithelial breaks between CLE+ and CLE- patients before (baseline) and after food challenge. Data are presented as means ± SE. exp, exposure.
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6. Figure 5
Intervillous space at baseline as visualized with endomicroscopy and scanning electron microscopy.
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7. Figure 6
Clinical response to customized exclusion diet after food challenge. The solid line represents the progress of CLE+, and the broken line represents the progress of CLE- patients during 1 year of follow-up evaluation. Data are shown as means ± SE.
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8. Supplementary Figure 1 Overview of study outline.
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9. Supplementary Figure 2
Comparison of total symptom score evaluated by visual analogue scales at baseline. Data shown as mean ± SE.
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10. Supplementary Figure 3
Baseline levels of intraepithelial lymphocytes comparing healthy controls with CLE+ and CLE- patients. Data shown as mean ± SE.
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11. Supplementary Figure 4
Comparison of intraepithelial lymphocytes at baseline and after exposure to water/simethicone in CLE- and CLE+ patients. Data are presented as mean SE.
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12. Supplementary Figure 5
(A/B) Comparison of intraepithelial lymphocytes at baseline and post exposure in CLE- (A) and CLE+ (B) patients. (C/D) Comparison of IEL after exposure to water and specific allergen in CLE- (C) and CLE+ (D) patients.
Gastroenterology  , e4DOI: ( /j.gastro )
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