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Dr J. Deschner, D. M. D. , Ph. D. , Dr B. Rath-Deschner, D. M. D. , Ph

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Presentation on theme: "Dr J. Deschner, D. M. D. , Ph. D. , Dr B. Rath-Deschner, D. M. D. , Ph"— Presentation transcript:

1 Regulation of matrix metalloproteinase expression by dynamic tensile strain in rat fibrochondrocytes 
Dr J. Deschner, D.M.D., Ph.D., Dr B. Rath-Deschner, D.M.D., Ph.D., Dr S. Agarwal, Ph.D.  Osteoarthritis and Cartilage  Volume 14, Issue 3, Pages (March 2006) DOI: /j.joca Copyright © 2005 OsteoArthritis Research Society International Terms and Conditions

2 Fig. 1 (A) Constitutive expression of mRNA for MMPs and TIMPs in fibrochondrocytes, as analyzed by end-point RT-PCR. Representative data from one of three experiments are shown. (B) MMP-9 and (C) MMP-13 mRNA expression in fibrochondrocytes at 4 and 24h, as determined by real-time PCR. Cells were treated with 1ng/ml of IL-1β and/or subjected to CTS at 20% and 0.05Hz. Results are shown as means±s.e.m. (n=6/group). For statistical analysis, the One-Way ANOVA and the post hoc multiple comparison Tukey's test were employed. *Significantly (P<0.05) different from all other groups including control, †significantly (P<0.05) different from IL-1β-treated cells, ‡significantly (P<0.05) different from IL-1β-treated cells in the presence and absence of CTS. Representative data from one of four experiments are shown. (D) Protein synthesis of MMP-3, -7, -8, -9, -13, -16, and -17 at 24h, as determined by Western blot analysis. Cells were treated with 1ng/ml of IL-1β and/or subjected to CTS at 20% and 0.05Hz for 24h. Representative gels from one of three experiments are shown. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2005 OsteoArthritis Research Society International Terms and Conditions

3 Fig. 2 Expression of mRNA for (A) MMP-3, (B) MMP-7, (C) MMP-8, (D) MMP-16, (E) MMP-17, and (F) MMP-19 in fibrochondrocytes at 24h, as determined by real-time PCR. Cells were treated with 1ng/ml of IL-1β and/or subjected to CTS at 20% and 0.05Hz. Results are shown as means±s.e.m. (n=6/group). For statistical analysis, the One-Way ANOVA and the post hoc multiple comparison Tukey's test were employed. *Significantly (P<0.05) different from all other groups including control, †significantly (P<0.05) different from IL-1β-treated cells, ‡significantly (P<0.05) different from IL-1β-treated cells in the presence and absence of CTS. Representative data from one of four experiments are shown. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2005 OsteoArthritis Research Society International Terms and Conditions

4 Fig. 3 Expression of mRNA for (A) MMP-9 and (B) MMP-13 at 24h, as analyzed by real-time RT-PCR. Fibrochondrocytes were stimulated with IL-1β for 24h while being subjected to CTS (20%, 0.05Hz) either only for the initial 1, 2, 4, 8, 12, 16, and 20h or for the entire 24h-interval. Results are shown as means±s.e.m. (n=6/group). For statistical analysis, the One-Way ANOVA and the post hoc multiple comparison Dunnett's test were employed. *Significantly (P<0.05) different from unstretched IL-1β-treated cells. Representative data from one of three experiments are shown. (C) MMP-9 and (D) MMP-13 mRNA expression at 24, 36, and 48h. Cells were incubated with 1ng/ml of IL-1β for 24, 36, and 48h, respectively, while CTS (20%, 0.05Hz) was applied only for the initial 8h of the entire incubation time. Results are shown as means±s.e.m., (n=6/group). For statistical analysis, the One-Way ANOVA and the post hoc multiple comparison Dunnett's test were employed. *Significantly (P<0.05) different from unstretched IL-1β-treated cells. Representative data from one of four experiments are shown. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2005 OsteoArthritis Research Society International Terms and Conditions

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