1. Volume 21, Issue 10, Pages 2706-2713 (December 2017)
Pervasive within-Mitochondrion Single-Nucleotide Variant Heteroplasmy as Revealed by Single-Mitochondrion Sequencing 
Jacqueline Morris, Young-Ji Na, Hua Zhu, Jae-Hee Lee, Hoa Giang, Alexandra V. Ulyanova, Gordon H. Baltuch, Steven Brem, H. Isaac Chen, David K. Kung, Timothy H. Lucas, Donald M. O’Rourke, John A. Wolf, M. Sean Grady, Jai-Yoon Sul, Junhyong Kim, James Eberwine 
Cell Reports 
Volume 21, Issue 10, Pages (December 2017)
DOI: /j.celrep
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2. Cell Reports 2017 21, 2706-2713DOI: (10.1016/j.celrep.2017.11.031)
Copyright © 2017 The Author(s) Terms and Conditions

3. Figure 1 Overview of Sample Collection and Processing
(A–F) Primary cultures of astrocytes (A–C) or neurons (D–F) loaded with MitoTracker Red with pre- and post- isolation images of target mitochondrion (red arrows) are shown in (B) versus (C) and (E) versus (F). Scale bars in full size images are 20 microns (A and D) and, in zoomed images, 5 microns (B, C, E, and F). Pixels in the zoomed images were resampled for clarity.
(G) Schematic of mtDNA coverage by round 1 and 2 PCR amplicons. Round 2 PCR reactions were pooled (G; pooled PCR) prior to acoustic shearing (G; sheared PCR) and standard Illumina library construction (G; DNA libraries).
See also Tables S1 and S2.
Cell Reports  , DOI: ( /j.celrep )
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4. Figure 2
Allele Frequencies Inherited from the Mother between Mouse Single-Mitochondrion Samples at High-Confidence Positions
(A) Sample lineage tree for single-mitochondrion samples of pups from mother IDs 4, 11, and 19 scaled to the number of SNVs per sample indicated by the key to the right.
(B) Histogram of read frequencies (10%–100%) of the most abundant non-reference alleles across all single mitochondrion (n = 118). The inset plots the log2 transformed frequencies against the minor allele frequencies.
(C) Dot plot of the most frequent non-reference allele frequency for all 118 single mouse mitochondrion for position 3,816, 9,027, or 9,461. Each dot represents up to 4 observations, each of which is color coded based on the mother ID.
See also Figure S1, Figure S2, and Figure S3.
Cell Reports  , DOI: ( /j.celrep )
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5. Figure 3
Relationship between Human Single Mitochondrion and Comparison to Mouse
(A) Image of human cell number 5 (scale bar, 100 microns). Pre- and post-isolation images and enlarged mitochondrion images shown adjacent (scale bar, 10 microns).
(B) Dot plot of arcsin transformed allele frequency deviations from means for human or mouse single mitochondrion. The top two panels show between cell deviations (human, n = 3 cells from 1 individual; mouse, n = 19 individuals each with 3, 2, 2, 9, 6, 3, 7, 3, 3, 3, 2, 4, 7, 2, 12, 8, 3, 4, or 2 cells), while the bottom panel shows within cell deviations (human, n = 3 cells each with 2, 5, or 9 mitochondrion [DF = 13]; mouse, n = 16 cells with 2, 2, 5, 2, 2, 3, 2, 2, 3, 2, 3, 2, 3, 2, 3, or 2 mitochondrion [DF = 24]).
See also Figure S3.
Cell Reports  , DOI: ( /j.celrep )
Copyright © 2017 The Author(s) Terms and Conditions