1. Both host and graft vessels contribute to revascularization of xenografted human ovarian tissue in a murine model 
Anne-Sophie Van Eyck, M.D., Caroline Bouzin, Pharm. D., Ph.D., Olivier Feron, Pharm. D., Ph.D., Lydia Romeu, B.Sc., Anne Van Langendonckt, Ph.D., Jacques Donnez, M.D., Ph.D., Marie-Madeleine Dolmans, M.D., Ph.D. 
Fertility and Sterility 
Volume 93, Issue 5, Pages (March 2010)
DOI: /j.fertnstert
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Double labeling demonstrating the species specificity of von Willebrand factor (vWF) and CD31 antibodies. (A–D) Human tissue (ovarian tissue): positive staining for vWF but absence of staining for CD31. (E–H) Mouse tissue (endometrium): positive staining for CD31 but no signal for vWF. Original magnification, ×100.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Histologic evaluation of frozen sections. (A) Fresh ovarian tissue showing stroma with a high cellular density. (B) Ovarian graft on day 3 with central necrosis, surrounded by stroma with a low cellular density. (B') Some follicles showed an eosinophilic cytoplasm and pyknotic granulosa cells (GC) on day 3. (C) On day 5, necrosis progressively disappeared from the center of the graft and healthy-looking follicles were observed at the periphery of the fragment. (D) By day 10, the cellular density of the stroma was lower than in fresh ovarian tissue but higher than on day 5. A close-up of a vessel is shown in D'. (A—D) Original magnification, ×100; scale, 100 μm; (B', D') Original magnification, ×400, scale, 25 μm.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Time course of reperfusion and revascularization processes. Perfused areas and murine endothelial areas increased progressively from day 3–day 5, and significantly by day 10 (Pa<0,001, Pb<0,05). Human vasculature was similar to fresh ovarian biopsies on days 3 and 5 after grafting. On day 10, a significant increase in human vasculature was observed compared with days 3 and 5 and to fresh biopsies (cP<.05; dP<.05). The sum of murine and human vessels significantly increased from day 3–day 10 (error propagation: ∗P<.05), with similar kinetics to perfusion (correlation coefficient: 0.86). bar: mean perfused area % ± SD (left Y axis); red line: mean human endothelial area ± SD; green line: mean murine endothelial area ± SD (right Y axis); interrupted black line: sum of mean human and murine endothelial area; gray band: mean human endothelial area observed on six fresh ovarian cortical biopsies with a 95% confidence interval.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
Spatial distribution of vascular parameters. Perfusion and murine vessels increased progressively between days 3 and 5 (∗P=.067, ∗∗P=.084) and significantly by day 10 (∗∗∗P<.003) from the periphery to the center of the graft. Native human vessels persisted on days 3 and 5. They were mainly located at the periphery of the fragment. A significant increase in human vasculature was observed on day 10 (∗∗∗∗P<.05), predominantly in the intermediate and central zones.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

6. Figure 5
Illustration of vascular and perfusion parameters on day 5 after grafting. (A) Double immunostaining of human and murine vascular structures using species-specific antibodies (merge image of green spectrum and red spectrum; red: von Willebrand factor human-specific endothelial cell marker; green: CD31 mouse-specific endothelial cell marker). (B) Graft blood vessels perfused at the time of Hoechst injection appear in blue. Hoechst uptake was present at the periphery of the graft but absent in the center. (C) Merged pictures of A and B represent the vascular structures perfused at the time of Hoechst injection. At the periphery of the graft, a perfused area (blue staining) was colocalized with murine neovessels and some human vessels. Nonperfused human vessels were present in the center of the graft. (D) Enlargement of the perfused vessels, C. The Hoechst stained nuclei of endothelial cells and the first cells found around the vessels perfused at the time of injection. P = periphery of the graft; C = center of the graft.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions