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Regression of pressure-induced left ventricular hypertrophy is characterized by a distinct gene expression profile William E. Stansfield, MD, Peter C. Charles, PhD, Ru-hang Tang, PhD, Mauricio Rojas, MD, Rajendra Bhati, MD, Nancy C. Moss, MD, Cam Patterson, MD, Craig H. Selzman, MD The Journal of Thoracic and Cardiovascular Surgery Volume 137, Issue 1, Pages e8 (January 2009) DOI: /j.jtcvs Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions
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Figure 1 Physiologic and structural evidence of left ventricular hypertrophy reversal. A, Heart weight (HW)/body weight (BW) ratios (n = 11–22 per group) and serial echocardiographic measurements showing changes over the depicted time period (n = 11–22 per group). B, Left ventricular (LV) cross-sections stained with periodic acid–Schiff. C, Calculated cardiomyocyte cross-sectional areas (n = 4 per group). ∗P < .05 versus the sham group, †P < .05 versus the band group. The Journal of Thoracic and Cardiovascular Surgery , e8DOI: ( /j.jtcvs ) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions
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Figure 2 Gene expression associated with left ventricular hypertrophy regression. A, Venn diagram depicts different and shared genes in each experimental contrast (P < .01 and absolute fold change >1.2). B, Most differentially expressed genes in the regression contrast (deband vs band). C, Western Blot for selected proteins identified in the deband versus band comparison. D, Unsupervised gene clustering between groups. B, Band; S, sham; DB, deband; SDB, sham deband. The Journal of Thoracic and Cardiovascular Surgery , e8DOI: ( /j.jtcvs ) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions
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Gene ontology clustering of significant genes between respective groups, as assessed by using DAVID Numbers represent the percentage of our significantly expressed genes in a particular cluster compared with the total number of significant genes. Individual genes might be representative of more than 1 gene ontologic category (thus total percentages will not add to 100%). The Journal of Thoracic and Cardiovascular Surgery , e8DOI: ( /j.jtcvs ) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions
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