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Escherichia coli DNA Contamination in AmpliTaq Gold Polymerase Interferes with TaqMan Analysis of lacZ  Jonna K Koponen, Anna-Mari Turunen, Seppo Ylä-Herttuala 

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1 Escherichia coli DNA Contamination in AmpliTaq Gold Polymerase Interferes with TaqMan Analysis of lacZ  Jonna K Koponen, Anna-Mari Turunen, Seppo Ylä-Herttuala  Molecular Therapy  Volume 5, Issue 3, Pages (March 2002) DOI: /mthe Copyright © 2002 American Society for Gene Therapy Terms and Conditions

2 Fig. 1 Amplification plots from lacZ real-time PCR. +SAMPLE (duplicates in blue and pink) indicates amplification plots from a known positive sample, - RT (duplicates in orange and yellow) indicates amplification plots from a sample where reverse transcriptase enzyme was omitted, and NTC (duplicates in red and green) indicates amplification plots from the amplification mix without template. Representative results are shown from TaqMan analyses repeated several times with different lots of AmpliTaq Gold DNA polymerase. Molecular Therapy 2002 5, DOI: ( /mthe ) Copyright © 2002 American Society for Gene Therapy Terms and Conditions


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