1. Mixed hematopoietic chimerism induces long-term tolerance to cardiac allografts in miniature swine 
Margaret L Schwarze, MD, Matthew T Menard, MD, Yasushi Fuchimoto, MD, Christene A Huang, PhD, Stuart Houser, MD, Kwabena Mawulawde, MD, Kenneth S Allison, BA, David H Sachs, MD, Joren C Madsen, MD 
The Annals of Thoracic Surgery 
Volume 70, Issue 1, Pages (July 2000)
DOI: /S (00)

2. Fig 1
Origin of available SLA haplotypes in partially inbred miniature swine. (SLA = swine leukocyte antigen.)
The Annals of Thoracic Surgery  , DOI: ( /S (00) )

3. Fig 2
Experimental protocols. (A) Timeline for the nonmyeloablative conditioning regimen which included WBI and TI. (B) Timeline for the nonmyelosuppressive conditioning regimen in which WBI is eliminated. ∗Note that swine #13235 did not receive CyA. (CyA = cyclosporine; TI = thymic irradiation; WBI = whole-body irradiation.)
The Annals of Thoracic Surgery  , DOI: ( /S (00) )

4. Fig 3
Donor lymphoid chimerism in mixed hematopoietic chimeras. The two-color scatter plots demonstrate mononuclear cell staining with FITC-conjugated anti-CD3 antibody versus biotin-conjugated PAA antibody. Double-stained cells (upper right quadrant of each scatter plot) represent PAA-positive T lymphocytes of donor origin. Percentage lymphocyte chimerism denotes the number of double positive stained cells over the total number of mononuclear cells acquired by FACS. (A) Representative scatter plot from recipient #13131 (WBI/TI and pCD3–CRM9 conditioning regimen) 13 days before cardiac transplantation and 126 days following cardiac ransplantation. (B) Representative scatter plot from recipient #12963 (TI and pCD3–CRM9 conditioning regimen) on the day of cardiac transplantation (postoperative day 0) and 116 days following cardiac transplantation. (pCD3–CRM9 = porcine CD3 immunotoxin; FACS = fluorescent activated cell sorter; FITC = fluorescein isothiocyanate; PAA = pig allelic antigen; TI = thymic irradiation; WBI = whole-body irradiation.)
The Annals of Thoracic Surgery  , DOI: ( /S (00) )

5. Fig 4
Three donor heart autopsy specimens stained with hematoxylin and eosin. (A) Section from recipient #10607 scored as ISHLT grade 4 rejection shows no evidence of intimal proliferation (magnification, 200×). (B) Section from recipient #12757 scored as ISHLT grade 1A rejection shows no evidence of intimal proliferation (magnification, 400×). (C) Section from recipient #13131 scored as ISHLT grade 0 rejection but shows evidence of mild intimal proliferation within intramyocardial arteries (magnification, 400×). (ISHLT = International Society for Heart and Lung Transplantation.)
The Annals of Thoracic Surgery  , DOI: ( /S (00) )