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SUPRA-NATIONAL REFERENCE LABORATORY NETWORK
DST QA Round 11, 2004 A. Van Deun & F. Portaels
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CONDUCT OF ROUND 11 20 strains, 10 in duplicate: total 30 cultures
non-MDR series: R-mono 7 strains from Borstel, 6 from Paris SRL pre-testing 7 Antwerp strains MIC, sequencing exclusion doubtful strains mass culture from single colony 0 contamination, 9 growth failures (#3, #9)
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LABS AND METHODS, Rd 6 TO 11
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JUDICIAL RESULTS OF ROUND 11
INH strains: 10 resistant, 10 susc. cultures: 15 resistant, 15 susc. agreement: 100% strains 98% strains
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JUDICIAL RESULTS OF ROUND 11
RIFAMPICIN 10 resistant, 10 susceptible strains 16 resistant, 14 susceptible cultures agreement: 100% strains 57% (judicial susc.) 1 strain (#6)
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JUDICIAL RESULTS OF ROUND 11
STREPTOMYCIN 11 resistant, 9 susceptible strains 16 resistant, 14 susceptible cultures agreement: 100% strains 96% strains 91% strain 70% strains (#1, 7) <70% strains (#6, 9)
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JUDICIAL RESULTS OF ROUND 11
ETHAMBUTOL strains: 5 resistant, 15 susc. cultures: 7 resistant, 23 susc. agreement: 100% strains 90-99% strains 87% strain 70-80% strains (#8, 11, 12)
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RESULTS OF ROUND 11
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RESULTS OF ROUND 11
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RIFAMPICIN INTERMEDIATE RESISTANT STRAIN: results by method
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COMPARISON PHENO / GENOTYPIC (SEQUENCING)
Results known for 7 Antwerp strains only Perfect correlation for H and E Poor correlation for S (rpsL, rrs530, rrs912) 0/3 resistant have mutation Perfect correlation for R with exception of the discordant strain leu533pro mutation majority finds susceptible
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RIFAMPICIN PROBLEM STRAIN MICs
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RIFAMPICIN PROBLEM STRAIN: CLINICALLY
#6, judicial HS resistant, 57% R-susceptible patient from Bujumbura, Burundi failure of 2EHRZ / 4H3R3 failure of 2SEHRZ / 1EHRZ / 5E3H3R3
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