1. Structural Biophysics, Niels Bohr Institute
Exercise on data analysis of Small-angle scattering data from Bovine Serum Albumin in solution
Lise Arleth, Professor
Structural Biophysics, Niels Bohr Institute
University of Copenhagen, Faculty of Life Sciences 1

2. Non-trivial structure/shape!
Bovine serum albumin
M = Da
Non-trivial structure/shape! 2

3. Exercise: SAXS analysis of Bovine Serum Albumin (BSA)
Use Svergun’s PRIMUS
-Plot data
-Make background subtractions
-Normalize by sample concentration – what do you see?
-Make Guinier plots and determine Radius of Gyration for the different concentrations – what do you see? Try to explain
-Make Indirect Fourier Transform using GNOM. Discuss.
What would be the next steps?
Next steps: Kør dammin53qw.exe on 4 mg/ml sample and try to determine the shape.