1. Effect of filaggrin breakdown products on growth of and protein expression by Staphylococcus aureus 
Helen Miajlovic, PhD, Padraic G. Fallon, PhD, Alan D. Irvine, MD, Timothy J. Foster, PhD 
Journal of Allergy and Clinical Immunology 
Volume 126, Issue 6, Pages e3 (December 2010)
DOI: /j.jaci
Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2. Fig 1
Effect of filaggrin breakdown products on growth of S aureus. Strain SH1000 was grown in TSB (A; ●) and RPMI (B; ●). UCA and PCA, 10 mmol/L (◊), were added to the growth media and to a control culture in which pH was neutralized after addition of UCA and PCA (○). Cell density of cultures was recorded at the indicated time points.
Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci )
Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3. Fig 2
Effect of filaggrin breakdown products on protein expression by S aureus. Strain SH1000 was grown in TSB, TSB supplemented with 10 mmol/L UCA and/or PCA, and TSB in which pH was neutralized after addition of UCA and PCA. Cell wall (A) and secreted protein fractions (B) were separated on 10% SDS-PAGE gels and visualized by Coomassie staining.
Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci )
Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4. Fig 3
Expression of proteins involved in skin colonization and inflammation. SH1000 was grown in TSB, TSB supplemented with 10 mmol/L UCA and/or PCA, and TSB in which pH was neutralized after addition of UCA and PCA. Cell wall proteins were isolated, separated by SDS-PAGE, and electroblotted onto PVDF membranes. Membranes were probed with anti-ClfB antibody, biotin-labeled fibronectin, HRP-conjugated IgG, and anti-SdrB repeat antibody.
Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci )
Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5. Fig 4
Effect of filaggrin breakdown products on expression of IsdA by S aureus. SH1000 was grown in RPMI, RPMI supplemented with 10 mmol/L UCA and PCA, and RPMI in which pH was neutralized after addition of UCA and PCA. Cell wall proteins were isolated, separated by SDS-PAGE, and electroblotted onto PVDF membranes. Membranes were probed with anti-IsdA antibody.
Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci )
Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6. Fig 5
Adherence of S aureus to host molecules. SH1000 was grown in TSB (●), TSB supplemented with 10 mmol/L UCA and PCA (□), and TSB in which pH was neutralized after addition of UCA and PCA (■). SH1000 cultures adjusted to OD600 of 1.0 were added to cytokeratin 10–coated (A) and fibronectin (Fn)–coated (B) microtiter plates. After incubation at 37°C, adherent cells were detected by crystal violet staining.
Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci )
Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7. Effect of acid on growth of S aureus
Effect of acid on growth of S aureus. Strain SH1000 was grown in TSB (pH 7; ●) and TSB adjusted to pH 5.5 (◊). Cell density of cultures was recorded at the indicated time points.
Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci )
Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8. Expression of proteins involved in skin colonization and inflammation
Expression of proteins involved in skin colonization and inflammation. SH1000 was grown in TSB (pH 7) and TSB adjusted to pH 5.5. Cell wall proteins were isolated, separated by SDS-PAGE, and electroblotted onto PVDF membranes. Membranes were probed with anti-ClfB antibody, biotin-labeled fibronectin, and HRP-conjugated IgG.
Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci )
Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

9. Adherence of S aureus to cytokeratin 10 and fibronectin
Adherence of S aureus to cytokeratin 10 and fibronectin. SH1000 was grown in TSB (pH 7 ●) and TSB adjusted to pH 5.5 (□). SH1000 cultures were adjusted to OD600 of 1.0 and added to cytokeratin 10–coated (A) and fibronectin-coated (B) microtiter plates. After incubation at 37°C, adherent cells were detected by crystal violet staining.
Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci )
Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions