1. Embryonic Fate Mapping Uncovers the Critical Role of microRNAs in the Development of Epidermal γδ T Cells 
Yi Yao, Queping Liu, Carly Martin, Congcong Yin, Zheng Dong, Qing-Sheng Mi, Li Zhou 
Journal of Investigative Dermatology 
Volume 138, Issue 1, Pages (January 2018)
DOI: /j.jid
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2. Figure 1
Fate mapping of resident γδ T cells using Csf1rCre reporter mice. (a–c) Skin and thymus from Csf1rCreRosa26miR-155-EGFP embryos were isolated at indicated time points (epidermis for E18.5 and P0). (b) EGFP expression was analyzed on Vγ3+ γδ T cells within CD45+ skin cells (a) or on total γδ T cells and Vγ3+ γδ T cells within live thymocytes. (c) Frequency of Vγ3+ γδ T cells in the skin/epidermis and thymus. (d) Epidermis and thymus were harvested from Csf1rCreRosa26EGFP embryos at E18.5. EGFP expression was analyzed on epidermal CD11b−γδTCR+ cells and on thymic total γδ T cells and Vγ3+γδ T cells. Data shown are representative of three independent experiments. Bars represent mean (n = 4–8/group). **P < EGFP, enhanced green fluorescent protein.
Journal of Investigative Dermatology  , DOI: ( /j.jid )
Copyright © 2017 The Authors Terms and Conditions

3. Figure 2
MiRNAs are required for epidermal γδ T-cell embryonic development. (a) Epidermis and (b–e) thymus were isolated from the Csf1rCreDicer embryos at E16.5 (whole skin), E18.5, and P0. Data shown are (a) the frequency of Vγ3+ γδ T cells within CD45+ cells, (b) the number of thymocytes, the frequency and number of total γδ T and Vγ3+ γδ T cells within thymocytes, (d) the frequency of Ki-67+ and annexin V+ Vγ3+γδ T cells (c), the frequency of CD122+, CD45RB+, and CD24+ Vγ3+ γδ T cells, and (e) the frequency of CCR4+, CCR10+, and S1P1+ Vγ3+γδ T cells as well as the staining for their corresponding isotype controls. Bars represent mean (n = 4–17/group from three independent experiments). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < N.S., not significant.
Journal of Investigative Dermatology  , DOI: ( /j.jid )
Copyright © 2017 The Authors Terms and Conditions