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Workflow of a sample-to-answer AST performed in less than 30 min

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Presentation on theme: "Workflow of a sample-to-answer AST performed in less than 30 min"— Presentation transcript:

1 Fig. 5. Workflow of a sample-to-answer AST performed in less than 30 min.
Workflow of a sample-to-answer AST performed in less than 30 min. (A) A clinical UTI sample was added to media with and without cip and incubated for 15 min. (B) During the antibiotic exposure step, the optimized bulk LAMP assay was performed on NAs prepared from an aliquot of the urine sample. Amplification indicated the presence of E. coli at clinically relevant concentrations. (C) Aliquots of the control and antibiotic-treated samples were added to extraction buffer, NAs were prepared for quantification using dLAMP, and samples were rapidly partitioned using SlipChips. (D) dLAMP was monitored in real time, and a susceptibility call was determined after 6.7 min of amplification; data for one resistant and one susceptible sample are shown. P values were calculated using a Z test (see Statistical analysis). Gray lines represent 95% confidence intervals. Nathan G. Schoepp et al., Sci Transl Med 2017;9:eaal3693 Published by AAAS

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